PMID- 8186413
OWN - NLM
STAT- MEDLINE
DCOM- 19940621
LR  - 20200315
IS  - 0916-8478 (Print)
IS  - 0916-8478 (Linking)
VI  - 38
IP  - 4
DP  - 1993 Dec
TI  - Construction of radiation-reduced hybrids and their use in mapping of microclones 
      from chromosome 10p11.2-q11.2.
PG  - 361-70
AB  - Radiation-reduced hybrids for mapping of DNA markers in the pericentromeric 
      region of chromosome 10 were developed. A Chinese hamster/human somatic cell 
      hybrid (762-8A) carrying chromosomes 10 and Y as the only human material were 
      exposed to 40,000 rads of irradiation and then rescued by fusion with 
      non-irradiated recipient Chinese hamster cells (GM459). Southern hybridization 
      analyses revealed that 10 of 128 HAT-resistant clones contained human chromosomal 
      fragments corresponding to at least one marker locus between FNRB (10p-11.2) and 
      RBP3 (10q11.2). These hybrids were then used to map micro-dissection clones 
      previously isolated and roughly mapped to this chromosomal region by fluorescence 
      in situ hybridization (FISH). Two of the six microclones studied could be mapped 
      to the proximity of the D10-S102 locus. These radiation hybrids are useful for 
      the construction of refined genetic maps of the pericentromeric region of 
      chromosome 10.
FAU - Fujita, S
AU  - Fujita S
AD  - Department of Medical Genetics, Osaka University Medical School, Japan.
FAU - Shin, E
AU  - Shin E
FAU - Nakamura, T
AU  - Nakamura T
FAU - Kurahashi, H
AU  - Kurahashi H
FAU - Kaneda, Y
AU  - Kaneda Y
FAU - Tanaka, K
AU  - Tanaka K
FAU - Mori, T
AU  - Mori T
FAU - Takai, S
AU  - Takai S
FAU - Nishisho, I
AU  - Nishisho I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Hum Genet
JT  - The Japanese journal of human genetics
JID - 9213239
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Line
MH  - Chromosome Mapping/*methods
MH  - *Chromosomes, Human, Pair 10
MH  - Cricetinae
MH  - Cricetulus
MH  - Humans
MH  - *Hybrid Cells/radiation effects
MH  - In Situ Hybridization, Fluorescence
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1007/BF01907982 [doi]
PST - ppublish
SO  - Jpn J Hum Genet. 1993 Dec;38(4):361-70. doi: 10.1007/BF01907982.