PMID- 8188747
OWN - NLM
STAT- MEDLINE
DCOM- 19940622
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 125
IP  - 4
DP  - 1994 May
TI  - Large-scale co-aggregation of fluorescent lipid probes with cell surface 
      proteins.
PG  - 795-802
AB  - Large scale aggregation of fluorescein-labeled immunoglobulin E (IgE) receptor 
      complexes on the surface of RBL cells results in the co-aggregation of a large 
      fraction of the lipophilic fluorescent probe 3,3'-dihexadecylindocarbocyanine 
      (diI) that labels the plasma membranes much more uniformly in the absence of 
      receptor aggregation. Most of the diI molecules that are localized in patches of 
      aggregated receptors have lost their lateral mobility as determined by 
      fluorescence photobleaching recovery. The diI outside of patches is mobile, and 
      its mobility is similar to that in control cells without receptor aggregates. It 
      is unlikely that the co-aggregation of diI with IgE receptors is due to specific 
      interactions between these components, as two other lipophilic probes of 
      different structures are also observed to redistribute with aggregated IgE 
      receptors, and aggregation of two other cell surface antigens also results in the 
      coredistribution of diI at the RBL cell surface. Quantitative analysis of CCD 
      images of labeled cells reveals some differences in the spatial distributions of 
      co-aggregated diI and IgE receptors. The results indicate that cross-linking of 
      specific cell surface antigens causes a substantial change in the organization of 
      the plasma membrane by redistributing pre-existing membrane domains or causing 
      their formation.
FAU - Thomas, J L
AU  - Thomas JL
AD  - Department of Physics, Cornell University, Ithaca, New York 14853.
FAU - Holowka, D
AU  - Holowka D
FAU - Baird, B
AU  - Baird B
FAU - Webb, W W
AU  - Webb WW
LA  - eng
GR  - AI18306/AI/NIAID NIH HHS/United States
GR  - AI22449/AI/NIAID NIH HHS/United States
GR  - T32 GM07273/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (3,3'-dihexadecylindocarbocyanine)
RN  - 0 (Carbocyanines)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Receptors, IgE)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Carbocyanines/*metabolism
MH  - Cell Line
MH  - Cell Membrane/metabolism
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes/*metabolism
MH  - *Lipid Metabolism
MH  - Receptors, IgE/*metabolism
PMC - PMC2120070
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
PMCR- 1994/11/02
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
PHST- 1994/11/02 00:00 [pmc-release]
AID - 94245799 [pii]
AID - 10.1083/jcb.125.4.795 [doi]
PST - ppublish
SO  - J Cell Biol. 1994 May;125(4):795-802. doi: 10.1083/jcb.125.4.795.