PMID- 8188929
OWN - NLM
STAT- MEDLINE
DCOM- 19940621
LR  - 20190909
IS  - 0303-6987 (Print)
IS  - 0303-6987 (Linking)
VI  - 21
IP  - 1
DP  - 1994 Feb
TI  - Interphase cytogenetics of melanocytic neoplasms: numerical aberrations of 
      chromosomes can be detected in interphase nuclei using centromeric DNA probes.
PG  - 1-6
AB  - This study shows that fluorescence in situ hybridization (FISH) to thin sections 
      cut from paraffin-embedded material can be used to distinguish between groups of 
      melanocytic neoplasms and thus may be useful as an investigational and diagnostic 
      tool. FISH with a probe for a repeated, alpha satellite sequence specific to 
      chromosome 17 was used to investigate the chromosomal composition of dysplastic 
      (or Clark's nevus) and Spitz's nevi and malignant melanomas. Hybridization was to 
      thin (approximately 6 microns) sections cut from paraffin blocks. The number of 
      signals per nucleus in normal diploid cells is expected to be less than 2 since 
      the sections are thinner than one nuclear diameter. Keratinocytes and lymphocytes 
      in these same sections showed 1-2 signals per nucleus with a mean of 1.2. 
      Dysplastic nevi showed 1-4 hybridization signals per nucleus with a mean of 1.5. 
      Spitz's nevi showed 1-2 signals per nucleus with a mean of 1.3. Melanomas showed 
      1-6 signals per nucleus with a mean of 2.1. We were thus able to use FISH to 
      demonstrate differences in chromosome numbers between groups of benign and 
      malignant melanocytic neoplasms. Technical improvements in the near future can be 
      expected to result in more precise estimates of chromosomal number.
FAU - Matsuta, M
AU  - Matsuta M
AD  - Department of Dermatology, Iwate Medical University, Japan.
FAU - Matsuta, M
AU  - Matsuta M
FAU - Kon, S
AU  - Kon S
FAU - Thompson, C
AU  - Thompson C
FAU - LeBoit, P E
AU  - LeBoit PE
FAU - Weier, H U
AU  - Weier HU
FAU - Gray, J W
AU  - Gray JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
RN  - 0 (DNA Probes)
SB  - IM
MH  - Centromere/*chemistry
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human, Pair 17
MH  - DNA Probes
MH  - Dysplastic Nevus Syndrome/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase/*genetics
MH  - Melanoma/*genetics
MH  - Nevus/*genetics
MH  - Nevus, Epithelioid and Spindle Cell/genetics
MH  - Skin Neoplasms/*genetics
EDAT- 1994/02/01 00:00
MHDA- 1994/02/01 00:01
CRDT- 1994/02/01 00:00
PHST- 1994/02/01 00:00 [pubmed]
PHST- 1994/02/01 00:01 [medline]
PHST- 1994/02/01 00:00 [entrez]
AID - 10.1111/j.1600-0560.1994.tb00683.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 1994 Feb;21(1):1-6. doi: 10.1111/j.1600-0560.1994.tb00683.x.