PMID- 8189035
OWN - NLM
STAT- MEDLINE
DCOM- 19940623
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 42
IP  - 6
DP  - 1994 Jun
TI  - Expression of mRNA for IL1 beta, IL6 and TGF beta 1 in developing human bone and 
      cartilage.
PG  - 733-44
AB  - Using in situ hybridization, we investigated the expression of mRNA for 
      interleukin-1 beta (IL1 beta), interleukin-6 (IL6), and transforming growth 
      factor-beta-1 (TGF beta 1) in sections of developing bone in human osteophytes. 
      The expression was related to the cellular activity of alkaline phosphatase to 
      aid in the identification of pre-osteoblast populations. IL1 beta mRNA was 
      localized in active osteoblasts within distinct areas of intramembranous 
      ossification. However, the expression was sporadic and appeared to occur at a 
      specific stage of the osteoblast life cycle. There was no IL1 beta mRNA 
      expression in any cell types during endochondral ossification. IL6 mRNA 
      expression was located within pre-osteoblasts and in newly differentiated and 
      matrix-secreting osteoblasts; expression was absent or reduced in flattened, 
      inactive osteoblasts. Weak or no IL6 expression was observed in chondroblasts and 
      chondrocytes, respectively. However, there was a close association between IL6 
      mRNA expression and the differentiation of mesenchymal cells into osteoblasts. 
      TGF beta 1 expression was localized to osteoblasts apposed to bone or cartilage 
      matrix; the intensity of expression correlated with matrix secretion. 
      Chondroblasts and chondrocytes expressed lower but significant levels of TGF beta 
      1 mRNA; the expression was lost with the progression to calcifying cartilage. The 
      three cytokines studied were differentially expressed both temporally and 
      spatially, suggesting different roles for each in osteoblast and chondrocyte 
      function.
FAU - Dodds, R A
AU  - Dodds RA
AD  - Bath Institute for Rheumatic Diseases, United Kingdom.
FAU - Merry, K
AU  - Merry K
FAU - Littlewood, A
AU  - Littlewood A
FAU - Gowen, M
AU  - Gowen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Biomarkers)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/analysis/metabolism
MH  - Biomarkers/analysis
MH  - Bone and Bones/*metabolism
MH  - Cartilage, Articular/*metabolism
MH  - Femur
MH  - *Gene Expression
MH  - Hip Prosthesis
MH  - Humans
MH  - In Situ Hybridization
MH  - Interleukin-1/*biosynthesis
MH  - Interleukin-6/*biosynthesis
MH  - Osteoarthritis/*metabolism/surgery
MH  - Osteoblasts/*metabolism
MH  - RNA Probes
MH  - RNA, Messenger/*biosynthesis
MH  - Transforming Growth Factor beta/*biosynthesis
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.1177/42.6.8189035 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1994 Jun;42(6):733-44. doi: 10.1177/42.6.8189035.