PMID- 8189261
OWN - NLM
STAT- MEDLINE
DCOM- 19940620
LR  - 20061115
IS  - 0022-3085 (Print)
IS  - 0022-3085 (Linking)
VI  - 80
IP  - 6
DP  - 1994 Jun
TI  - Expression and localization of messenger RNA and protein for monocyte 
      chemoattractant protein-1 in human malignant glioma.
PG  - 1056-62
AB  - Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell 
      lines and surgical specimens was studied by Northern blot analysis, 
      reverse-transcription polymerase chain reaction, in situ hybridization, and 
      immunohistochemistry. The samples tested consisted of 11 human glioma cell lines 
      and eight specimens of human malignant glioma (seven from glioblastomas and one 
      from a malignant ependymoma). Messenger ribonucleic acid (mRNA) of MCP-1 was 
      detected by either Northern blot or reverse-transcription polymerase chain 
      reaction analysis in all cell lines and tumor specimens examined. In vivo 
      expression of MCP-1 mRNA and protein was found predominantly in glioma cells with 
      large and pleomorphic nuclei rather than in areas of small nucleated glioma 
      cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or 
      protein. Tumor vessels with endothelial proliferation expressed a moderate level 
      of MCP-1 protein. Macrophages were found among the glioma cells, and the degree 
      of macrophage infiltration was grossly correlated with the level of MCP-1 
      expression. The study results suggest that MCP-1 produced by the glioma cells may 
      mediate macrophage infiltration into the glioma tissue.
FAU - Takeshima, H
AU  - Takeshima H
AD  - Department of Neurosurgery, Kumamoto University Medical School, Japan.
FAU - Kuratsu, J
AU  - Kuratsu J
FAU - Takeya, M
AU  - Takeya M
FAU - Yoshimura, T
AU  - Yoshimura T
FAU - Ushio, Y
AU  - Ushio Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosurg
JT  - Journal of neurosurgery
JID - 0253357
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Aged
MH  - Blotting, Northern
MH  - Brain Neoplasms/genetics/*metabolism/pathology
MH  - Chemokine CCL2
MH  - Chemotactic Factors/genetics/*metabolism
MH  - Child
MH  - Gene Expression
MH  - Glioma/genetics/*metabolism/pathology
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macrophages/pathology
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Tumor Cells, Cultured/metabolism
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
AID - 10.3171/jns.1994.80.6.1056 [doi]
PST - ppublish
SO  - J Neurosurg. 1994 Jun;80(6):1056-62. doi: 10.3171/jns.1994.80.6.1056.