PMID- 8190362
OWN - NLM
STAT- MEDLINE
DCOM- 19940620
LR  - 20190701
IS  - 0304-3940 (Print)
IS  - 0304-3940 (Linking)
VI  - 166
IP  - 1
DP  - 1994 Jan 17
TI  - Induction of c-fos mRNA expression in an in vitro hippocampal slice model of 
      adult rats after kainate but not gamma-aminobutyric acid or bicuculline 
      treatment.
PG  - 73-6
AB  - Levels of gene expression following in vitro treatment of rat hippocampal slices 
      with kainate, gamma-aminobutyric acid (GABA), or bicuculline were measured by the 
      reverse transcription-coupled polymerase chain reaction method. Following a 
      short-term exposure to kainate, c-fos gene expression was induced by 12-fold in 
      the adult, but not the newborn, hippocampus. Under the same experimental 
      conditions, zifl268 and brain-derived neurotrophic factor (BDNF) gene expression 
      were unchanged. Our results also demonstrate a lack of induction of c-fos, 
      zifl268 and BDNF after short-time treatment of either adult or newborn 
      hippocampal slices with GABA or bicuculline. The relevance of the differential 
      induction of gene expression in the adult and newborn in an in vitro hippocampal 
      slice model as compared to previously described in vivo models is discussed.
FAU - Massamiri, T
AU  - Massamiri T
AD  - Department of Pharmacology, University of California San Diego, La Jolla 92093.
FAU - Khrestchatisky, M
AU  - Khrestchatisky M
FAU - Ben-Ari, Y
AU  - Ben-Ari Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (RNA, Messenger)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - SIV03811UC (Kainic Acid)
RN  - Y37615DVKC (Bicuculline)
SB  - IM
GS  - c-fos
GS  - zif/268
MH  - Animals
MH  - Animals, Newborn
MH  - Bicuculline/*pharmacology
MH  - Gene Expression/*drug effects
MH  - *Genes, fos
MH  - Hippocampus/drug effects/*metabolism
MH  - In Vitro Techniques
MH  - Kainic Acid/*pharmacology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription, Genetic
MH  - gamma-Aminobutyric Acid/*pharmacology
EDAT- 1994/01/17 00:00
MHDA- 1994/01/17 00:01
CRDT- 1994/01/17 00:00
PHST- 1994/01/17 00:00 [pubmed]
PHST- 1994/01/17 00:01 [medline]
PHST- 1994/01/17 00:00 [entrez]
AID - 0304-3940(94)90843-5 [pii]
AID - 10.1016/0304-3940(94)90843-5 [doi]
PST - ppublish
SO  - Neurosci Lett. 1994 Jan 17;166(1):73-6. doi: 10.1016/0304-3940(94)90843-5.