PMID- 8193674
OWN - NLM
STAT- MEDLINE
DCOM- 19940628
LR  - 20191101
IS  - 1019-6773 (Print)
IS  - 1019-6773 (Linking)
VI  - 47
IP  - 2
DP  - 1993
TI  - 11-beta-Hydroxysteroid dehydrogenase of rat lung: enzyme kinetic, 
      oxidase-reductase ratio, electrolyte and trace element dependence.
PG  - 83-91
AB  - The modulation of the intracellular glucocorticoidal effect on surfactant 
      synthesis of the fetal lung by the metabolic capacity of 11 beta-hydroxysteroid 
      dehydrogenase (11 beta-HSD) could be an important factor in lung maturation. The 
      kinetic properties of microsomal 11 beta-HSD of the rat lung are characterized 
      with respect to product inhibition, substrate specificity, effect of electrolytes 
      or trace elements, and the dependence of the oxidase reductase (OR) ratio on 
      incubation conditions. With NADP+ product inhibition of the reductase was 
      demonstrated. The most common trace elements and electrolytes exhibited no effect 
      on the activity of 11 beta-HSD. It is shown that the OR ratio was strongly 
      dependent on assay conditions. With optimal assay conditions oxidase activity 
      exceeds reductase activity in adult and fetal rat lung microsomes (OR ratio > 1). 
      Thus, glucocorticoids are mainly metabolized to their inactive forms. The enzyme 
      activity in the adult is about 10 times higher than in the fetal lung. The low 
      enzyme activity in fetal lungs could be the reason why the glucocorticoidal 
      effects on surfactant synthesis are not suppressed despite the predominance of 
      oxidase activity.
FAU - Hundertmark, S
AU  - Hundertmark S
AD  - Department of Obstetrics and Gynecology, Klinikum Steglitz, Freie Universitat 
      Berlin, FRG.
FAU - Ragosch, V
AU  - Ragosch V
FAU - Schein, B
AU  - Schein B
FAU - Buhler, H
AU  - Buhler H
FAU - Fromm, M
AU  - Fromm M
FAU - Lorenz, U
AU  - Lorenz U
FAU - Weitzel, H K
AU  - Weitzel HK
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Enzyme Protein
JT  - Enzyme & protein
JID - 9422761
RN  - 53-59-8 (NADP)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Corticosterone/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Hydroxysteroid Dehydrogenases/drug effects/*metabolism
MH  - Lung/*enzymology
MH  - Male
MH  - Microsomes/*enzymology
MH  - NADP/metabolism/pharmacology
MH  - Oxidation-Reduction
MH  - Rats
MH  - Rats, Wistar
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1159/000468661 [doi]
PST - ppublish
SO  - Enzyme Protein. 1993;47(2):83-91. doi: 10.1159/000468661.