PMID- 8194042
OWN - NLM
STAT- MEDLINE
DCOM- 19940624
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 74
IP  - 1
DP  - 1994 May
TI  - Comparison of chromosome aberrations in leiomyoma and leiomyosarcoma using FISH 
      on archival tissues.
PG  - 19-24
AB  - Fluorescence in situ hybridization (FISH) with chromosome-specific probes was 
      used to study cytogenetic changes in five cases of leiomyosarcoma (LMS) and nine 
      cases of uterine leiomyoma (LM). Biotinylated DNA probes for the centromeric 
      regions of chromosomes 1, 6, 8, 9, 17, and 18, painting probes for chromosomes 1 
      and 22, and the cosmid probe for chromosome region 21q22.3 were used on nuclei 
      isolated from paraffin blocks. Four of five LMS cases revealed major chromosomal 
      aberrations, while the only case with minor clonal aberrations was subsequently 
      found not to be a typical LMS. The most common numerical aberrations found in the 
      LMS cases were extra copies of chromosome 8 (three of five cases), loss of 
      chromosome 1 (three of five cases), and loss of chromosome 6 (two of five cases). 
      One of two LMS cases studied with a chromosome 1 painting probe demonstrated 
      translocations of chromosome 1. In contrast to LMS, only five of nine uterine LM 
      cases had abnormal clones, and these were smaller than those in LMS. Two LM cases 
      showed 9% tetrasomy 8 with 17 or 20% monosomy 6, and three other cases had 
      monosomy 6 clones in 18-34% of cells. These results indicate that typical LMS is 
      characterized by multiple chromosomal aberrations affecting most of the cells, 
      whereas borderline LMS and LM have fewer affected chromosomes and less clonal 
      involvement.
FAU - Han, K
AU  - Han K
AD  - Department of Clinical Pathology, Catholic University Medical College, Seoul, 
      Korea.
FAU - Lee, W
AU  - Lee W
FAU - Harris, C P
AU  - Harris CP
FAU - Simsiman, R C
AU  - Simsiman RC
FAU - Lee, K
AU  - Lee K
FAU - Kang, C
AU  - Kang C
FAU - Meisner, L F
AU  - Meisner LF
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Probes)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leiomyoma/*genetics
MH  - Leiomyosarcoma/*genetics
MH  - Male
MH  - Middle Aged
MH  - Tissue Preservation
MH  - Uterine Neoplasms/genetics
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - 0165-4608(94)90023-X [pii]
AID - 10.1016/0165-4608(94)90023-x [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1994 May;74(1):19-24. doi: 10.1016/0165-4608(94)90023-x.