PMID- 8197118
OWN - NLM
STAT- MEDLINE
DCOM- 19940627
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 11
DP  - 1994 May 24
TI  - Fluid shear stress induces a biphasic response of human monocyte chemotactic 
      protein 1 gene expression in vascular endothelium.
PG  - 4678-82
AB  - The focal distribution of atherosclerotic lesions in the arterial tree is related 
      to the local shear stress generated by blood flow, but the molecular basis of the 
      atherogenic response of endothelial cells in these lesion-prone areas is still 
      unclear. We report that shear stress mediates a biphasic response of monocyte 
      chemotactic protein 1 (MCP-1) gene expression in vascular endothelial cells (EC). 
      Northern blot analysis indicated that the level of MCP-1 mRNA in human umbilical 
      vein EC (HUVEC) subjected to a shear stress of 16 dynes/cm2 (1 dyne = 10 microN) 
      for 1.5 hr increased by 2- to 3-fold when compared with static cells. The MCP-1 
      gene expression decreased to the basal level at 4 hr and then declined further to 
      become completely quiescent at 5 hr after the onset of shear. Once the gene 
      expression was fully suppressed, it remained quiescent even after static 
      incubation for 1.5 hr and would not respond to reshearing after this static 
      incubation. However, if the postshearing incubation extended from 1.5 to 24 hr, 
      the MCP-1 mRNA returned to the basal level and was then able to increase after 
      the reapplication of shear stress. Nuclear run-on experiments showed that the 
      shear-induced increased MCP-1 mRNA in HUVEC was regulated at the transcriptional 
      level. By using cycloheximide, it was shown that de novo protein synthesis was 
      not necessary for the induction of MCP-1 by shear stress. The biphasic response 
      of MCP-1 gene expression was found in experiments in which the applied shear 
      stress was 6, 16, or 32 dynes/cm2, and it was observed not only in HUVEC but also 
      in HeLa cells, glioma cell lines, and skin fibroblasts. This in vitro study 
      demonstrates that the response of MCP-1 gene to shear stress represents an 
      immediate early gene activation and suggests that this gene is probably 
      suppressed in EC that have been exposed to a constant shear stress.
FAU - Shyy, Y J
AU  - Shyy YJ
AD  - Institute for Biomedical Engineering, University of California at San Diego, La 
      Jolla 92093-0412.
FAU - Hsieh, H J
AU  - Hsieh HJ
FAU - Usami, S
AU  - Usami S
FAU - Chien, S
AU  - Chien S
LA  - eng
GR  - HL 19454/HL/NHLBI NIH HHS/United States
GR  - HL 43026/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
SB  - IM
MH  - Biomechanical Phenomena
MH  - Blotting, Northern
MH  - Cell Line
MH  - Chemokine CCL2
MH  - Chemotactic Factors/biosynthesis/*genetics
MH  - Endothelium, Vascular/cytology/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Protein Biosynthesis
MH  - RNA Processing, Post-Transcriptional
MH  - Transcription, Genetic
PMC - PMC43851
EDAT- 1994/05/24 00:00
MHDA- 1994/05/24 00:01
PMCR- 1994/11/24
CRDT- 1994/05/24 00:00
PHST- 1994/05/24 00:00 [pubmed]
PHST- 1994/05/24 00:01 [medline]
PHST- 1994/05/24 00:00 [entrez]
PHST- 1994/11/24 00:00 [pmc-release]
AID - 10.1073/pnas.91.11.4678 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 May 24;91(11):4678-82. doi: 
      10.1073/pnas.91.11.4678.