PMID- 8197126
OWN - NLM
STAT- MEDLINE
DCOM- 19940627
LR  - 20211001
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 11
DP  - 1994 May 24
TI  - Invasiveness and metastasis of NIH 3T3 cells induced by Met-hepatocyte growth 
      factor/scatter factor autocrine stimulation.
PG  - 4731-5
AB  - The met protooncogene product, Met, is the tyrosine kinase growth factor receptor 
      for hepatocyte growth factor/scatter factor (HGF/SF). NIH 3T3 cells express 
      HGF/SF endogenously and become tumorigenic in nude mice via an autocrine 
      mechanism when murine Met is expressed ectopically (Metmu cells) or when human 
      Met and human HGF/SF are coexpressed (HMH cells). Here, we show that Metmu and 
      HMH cells are invasive in vitro and display enhanced protease activity necessary 
      for the invasive phenotype. In experimental and spontaneous metastasis assays, 
      Metmu or HMH cells metastasize to the lung, but lower numbers of subcutaneously 
      injected Metmu and HMH cells produced invasive tumors in the heart, diaphragm, 
      salivary gland, and retroperitoneum. It has been reported elsewhere that Met 
      expression increased with tumor passage in athymic nude mice, and these tumor 
      explants show enhanced activity in the metastasis assays. Autocrine-mediated 
      Met-HGF/SF signal transduction in NIH 3T3 mesenchymal cells may provide an 
      important system for understanding the biological process of metastasis.
FAU - Rong, S
AU  - Rong S
AD  - Advanced BioScience Laboratories-Basic Research Program, National Cancer 
      Institute-Frederick Cancer Research and Development Center, MD 21702.
FAU - Segal, S
AU  - Segal S
FAU - Anver, M
AU  - Anver M
FAU - Resau, J H
AU  - Resau JH
FAU - Vande Woude, G F
AU  - Vande Woude GF
LA  - eng
GR  - N01-CO-74101/CO/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Proto-Oncogene Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 3.4.24.- (Collagenases)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Cell Movement
MH  - Cell Transformation, Neoplastic
MH  - Collagenases/metabolism
MH  - Hepatocyte Growth Factor/*physiology
MH  - Humans
MH  - Mice
MH  - Mice, Nude
MH  - *Neoplasm Invasiveness
MH  - *Neoplasm Metastasis
MH  - Phenotype
MH  - Proto-Oncogene Proteins/*physiology
MH  - Proto-Oncogene Proteins c-met
MH  - Receptor Protein-Tyrosine Kinases/*physiology
PMC - PMC43862
EDAT- 1994/05/24 00:00
MHDA- 1994/05/24 00:01
PMCR- 1994/11/24
CRDT- 1994/05/24 00:00
PHST- 1994/05/24 00:00 [pubmed]
PHST- 1994/05/24 00:01 [medline]
PHST- 1994/05/24 00:00 [entrez]
PHST- 1994/11/24 00:00 [pmc-release]
AID - 10.1073/pnas.91.11.4731 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 May 24;91(11):4731-5. doi: 
      10.1073/pnas.91.11.4731.