PMID- 8197590
OWN - NLM
STAT- MEDLINE
DCOM- 19940628
LR  - 20190819
IS  - 0300-483X (Print)
IS  - 0300-483X (Linking)
VI  - 89
IP  - 2
DP  - 1994 Apr 18
TI  - Cyanide-induced lipid peroxidation in different organs: subcellular distribution 
      and hydroperoxide generation in neuronal cells.
PG  - 127-37
AB  - To evaluate hydroperoxide generation as a potential mechanism of cyanide 
      neurotoxicity, mice were treated with KCN (7 mg/kg, subcutaneously (s.c.)) and 
      the level of lipid peroxidation (expressed as conjugated dienes) was measured 
      later in various organs. Brain showed elevated conjugated diene levels after 
      cyanide but the liver, which is not considered a target for cyanide toxicity, 
      showed no increase. The heart also showed no increase, whereas kidney conjugated 
      dienes slowly increased to a peak 1 h after cyanide. In vitro studies show 
      elevation of peroxidized lipids in mouse brain cortical slices following 
      incubation with KCN (0.1 mM). Omission of calcium from the medium or pretreatment 
      of brain slices with diltiazem (a calcium channel blocker) prevented formation of 
      conjugated dienes by KCN. Calcium thus appears to play a critical role in 
      cyanide-induced generation of peroxidized lipids in neuronal cells. Subcellular 
      fractionation of brains from mice treated with cyanide showed that lipid 
      peroxidation increased in the microsomal fraction but not in the mitochondrial 
      fraction. Fluorescent studies using 2,7-dichlorofluorescein (a hydroperoxide 
      sensitive fluorescent dye) show that hydroperoxides are generated rapidly after 
      cyanide treatment of PC12 cells, a neuron-like cell, and hydroperoxide levels 
      remain elevated for many minutes in the presence of cyanide. These results 
      suggest that hydroperoxide generation with subsequent peroxidation of lipids may 
      lead to changes in structure and function of certain membranes and contribute to 
      the neurotoxic damage produced by cyanide.
FAU - Ardelt, B K
AU  - Ardelt BK
AD  - Department of Pharmacology and Toxicology, Purdue University, West Lafayette, IN 
      47907-1334.
FAU - Borowitz, J L
AU  - Borowitz JL
FAU - Maduh, E U
AU  - Maduh EU
FAU - Swain, S L
AU  - Swain SL
FAU - Isom, G E
AU  - Isom GE
LA  - eng
GR  - ES04140/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Toxicology
JT  - Toxicology
JID - 0361055
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EE92BBP03H (Diltiazem)
RN  - MQD255M2ZO (Potassium Cyanide)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Brain/*drug effects/metabolism/ultrastructure
MH  - Calcium/physiology
MH  - Cell Line
MH  - Diltiazem/pharmacology
MH  - Heart/drug effects
MH  - Hydrogen Peroxide/*metabolism
MH  - Injections, Subcutaneous
MH  - Kidney/drug effects/metabolism
MH  - Lipid Peroxidation/*drug effects/physiology
MH  - Liver/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Microsomes/drug effects/metabolism
MH  - Mitochondria/drug effects/metabolism
MH  - Neurons/*drug effects/metabolism/ultrastructure
MH  - Potassium Cyanide/*toxicity
EDAT- 1994/04/18 00:00
MHDA- 1994/04/18 00:01
CRDT- 1994/04/18 00:00
PHST- 1994/04/18 00:00 [pubmed]
PHST- 1994/04/18 00:01 [medline]
PHST- 1994/04/18 00:00 [entrez]
AID - 0300-483X(94)90221-6 [pii]
AID - 10.1016/0300-483x(94)90221-6 [doi]
PST - ppublish
SO  - Toxicology. 1994 Apr 18;89(2):127-37. doi: 10.1016/0300-483x(94)90221-6.