PMID- 8200435
OWN - NLM
STAT- MEDLINE
DCOM- 19940707
LR  - 20091119
IS  - 0014-4886 (Print)
IS  - 0014-4886 (Linking)
VI  - 127
IP  - 1
DP  - 1994 May
TI  - Distribution of intracerebral ventricularly administered neurotrophins in rat 
      brain and its correlation with trk receptor expression.
PG  - 23-36
AB  - To assess the potential effectiveness by which injected neurotrophins can diffuse 
      throughout the brain, we used autoradiographic and immunohistochemical techniques 
      to examine the brain distributions of nerve growth factor (NGF), brain-derived 
      neurotrophic factor (BDNF), and neurotrophin-3 (NT-3) after a single injection 
      into the lateral cerebral ventricle (ICV) in rats. As described previously, 
      ICV-injected NGF labeled cholinergic neurons in the basal forebrain. Injection of 
      BDNF resulted in few or no labeled neurons in the basal forebrain or in the 
      substantia nigra. However, very intense labeling was associated with the 
      ventricular walls and immediate parenchyma. The distribution of NT-3 after ICV 
      injection was intermediate between that of NGF and BDNF. In the basal forebrain, 
      similar neurotrophin distributions were observed in neonate versus adult animals. 
      Our in situ hybridization analysis has shown that mRNA encoding the BDNF 
      receptor(s) (trkB) is highly expressed by ependymal cells as well as by many 
      neurons and glia. On the other hand, expression of the high-affinity NGF receptor 
      (trkA) is restricted to cholinergic neurons in basal forebrain and striatum. In 
      addition, staining with antisera specific for the trkA or trkB receptors 
      demonstrated that their expression patterns closely reflect their mRNA 
      distributions. Taken together, these data suggest that the presence of the trkB 
      receptor on the ependymal layer of the ventricle and its expression throughout 
      the brain parenchyma represents a significant impediment to the adequate 
      diffusion of ICV-injected BDNF into the brain for delivery to target neurons.
FAU - Yan, Q
AU  - Yan Q
AD  - Department of Neurobiology, Amgen, Inc., Amgen Center, Thousand Oaks, California 
      91320.
FAU - Matheson, C
AU  - Matheson C
FAU - Sun, J
AU  - Sun J
FAU - Radeke, M J
AU  - Radeke MJ
FAU - Feinstein, S C
AU  - Feinstein SC
FAU - Miller, J A
AU  - Miller JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Exp Neurol
JT  - Experimental neurology
JID - 0370712
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
GS  - trk
GS  - trkA
GS  - trkB
MH  - Animals
MH  - Brain/drug effects/*metabolism
MH  - Brain-Derived Neurotrophic Factor
MH  - Cerebral Ventricles/drug effects/*physiology
MH  - Gene Expression/*drug effects
MH  - Humans
MH  - Immunohistochemistry
MH  - Injections, Intraventricular
MH  - Nerve Growth Factors/administration & dosage/*pharmacology
MH  - Nerve Tissue Proteins/administration & dosage/*pharmacology
MH  - Neurons/drug effects/*metabolism
MH  - Neurotrophin 3
MH  - Prosencephalon/metabolism
MH  - Proto-Oncogene Proteins/analysis/*biosynthesis
MH  - Proto-Oncogenes
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor Protein-Tyrosine Kinases/analysis/*biosynthesis
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptor, trkA
MH  - Receptors, Growth Factor/analysis/*biosynthesis
MH  - Receptors, Nerve Growth Factor/analysis/*biosynthesis
MH  - Recombinant Proteins/administration & dosage/pharmacology
MH  - Substantia Nigra/metabolism
EDAT- 1994/05/01 00:00
MHDA- 1994/05/01 00:01
CRDT- 1994/05/01 00:00
PHST- 1994/05/01 00:00 [pubmed]
PHST- 1994/05/01 00:01 [medline]
PHST- 1994/05/01 00:00 [entrez]
AID - S0014-4886(84)71076-4 [pii]
AID - 10.1006/exnr.1994.1076 [doi]
PST - ppublish
SO  - Exp Neurol. 1994 May;127(1):23-36. doi: 10.1006/exnr.1994.1076.