PMID- 8202359
OWN - NLM
STAT- MEDLINE
DCOM- 19940701
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 22
IP  - 9
DP  - 1994 May 11
TI  - Intramolecular folding of a fragment of the cytosine-rich strand of telomeric DNA 
      into an i-motif.
PG  - 1600-6
AB  - In the recently discovered i-motif, four stretches of cytosine form two 
      parallel-stranded duplexes whose C.C+ base pairs are fully intercalated. The 
      i-motif may be recognized by characteristic Overhauser cross-peaks of the proton 
      NMR spectrum, reflecting short H1'-H1' distances across the minor groove, and 
      short internucleotide amino-proton-H2'/H2" across the major groove. We report the 
      observation of such cross-peaks in the spectra of a fragment of the C-rich 
      telomeric strand of vertebrates, d[CCCTAA]3CCC. The spectra also demonstrate that 
      the cytosines are base-paired and that proton exchange is very slow, as reported 
      previously for the i-motif. From UV absorbance and gel chromatography 
      measurements, we assign these properties to an i-motif which includes all or 
      nearly all the cytosines, and which is formed by intramolecular folding at 
      slightly acid or neutral pH. A fragment of telomeric DNA of Tetrahymena, 
      d[CCCCAA]3CCCC, has the same properties. Hence four consecutive C stretches of a 
      C-rich telomeric strand can fold into an i-motif. Hypothetically, this could 
      occur in vivo.
FAU - Leroy, J L
AU  - Leroy JL
AD  - l'URA D1254 du CNRS, Palaiseau, France.
FAU - Gueron, M
AU  - Gueron M
FAU - Mergny, J L
AU  - Mergny JL
FAU - Helene, C
AU  - Helene C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA, Protozoan)
RN  - 0 (Protons)
RN  - 8J337D1HZY (Cytosine)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Base Composition
MH  - Base Sequence
MH  - Chromatography, Gel
MH  - Cytosine/*chemistry
MH  - DNA/*chemistry
MH  - DNA, Protozoan/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Magnetic Resonance Spectroscopy
MH  - Molecular Sequence Data
MH  - *Nucleic Acid Conformation
MH  - Protons
MH  - Spectrophotometry, Ultraviolet
MH  - Telomere/*chemistry
MH  - Tetrahymena/*genetics
PMC - PMC308035
EDAT- 1994/05/11 00:00
MHDA- 1994/05/11 00:01
CRDT- 1994/05/11 00:00
PHST- 1994/05/11 00:00 [pubmed]
PHST- 1994/05/11 00:01 [medline]
PHST- 1994/05/11 00:00 [entrez]
AID - 10.1093/nar/22.9.1600 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1994 May 11;22(9):1600-6. doi: 10.1093/nar/22.9.1600.