PMID- 8202541
OWN - NLM
STAT- MEDLINE
DCOM- 19940706
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 91
IP  - 12
DP  - 1994 Jun 7
TI  - Expansion of CREB's DNA recognition specificity by Tax results from interaction 
      with Ala-Ala-Arg at positions 282-284 near the conserved DNA-binding domain of 
      CREB.
PG  - 5642-6
AB  - The transactivator protein of human T-lymphotropic virus type I (HTLV-I), Tax, 
      forms multiprotein complexes with the ubiquitous transcription factor CREB and 
      the CREB/ATF-1 heterodimer. The interaction between Tax and CREB is highly 
      specific and results in increased binding of the Tax/CREB complexes to the HTLV-I 
      21-bp repeats. Despite the extensive sequence similarities between CREB and 
      ATF-1, Tax interacts with ATF-1 only marginally. Compared with CREB, Tax/CREB 
      exhibits greatly increased DNA recognition specificity and preferentially 
      assembles on a consensus binding site, GGGGG(T/A)TGACG(T/C)(A/C)TA(T/C)C-CCCC, 
      homologous to the HTLV-I 21-bp repeats. Here we report that Tax affects CREB 
      binding to the Tax-inducible DNA elements by interacting with the basic-leucine 
      zipper (bZip) domain of CREB. We show by domain switching that the basic region 
      in CREB bZip can confer on c-Jun and ATF-1 leucine zippers the ability to 
      interact with Tax in vitro. Mutational analyses further demonstrate that the 
      amino acid residues of CREB critical for Tax/CREB interaction are Ala-Ala-Arg at 
      positions 282-284 (AAR284), immediately upstream of the highly conserved 
      DNA-binding domain (R/K)XX(R/K) N(R/K)XAAXX(S/C)RX(R/K)(K/R) characteristic of 
      all bZip proteins. Specific amino acid substitutions in AAR284 of CREB weakened 
      or abolished Tax/CREB interaction, whereas reciprocal changes in ATF-1 allowed it 
      to interact with Tax. These results support a model in which the specific 
      interaction between Tax and the AAR284 residues near the DNA-binding domain of 
      CREB results in a multiprotein complex with altered DNA recognition property. 
      This protein complex assembles selectively on the viral Tax-responsive 21-bp 
      repeats to augment transcription.
FAU - Adya, N
AU  - Adya N
AD  - Department of Medicine, Case Western Reserve University School of Medicine, 
      Cleveland, OH 44106-4984.
FAU - Zhao, L J
AU  - Zhao LJ
FAU - Huang, W
AU  - Huang W
FAU - Boros, I
AU  - Boros I
FAU - Giam, C Z
AU  - Giam CZ
LA  - eng
GR  - R01 CA48709/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Activating Transcription Factor 1)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gene Products, tax)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Activating Transcription Factor 1
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites
MH  - Consensus Sequence
MH  - Cyclic AMP Response Element-Binding Protein/*chemistry/metabolism
MH  - DNA-Binding Proteins/chemistry/metabolism
MH  - Gene Products, tax/*chemistry/metabolism
MH  - Human T-lymphotropic virus 1/genetics
MH  - Humans
MH  - In Vitro Techniques
MH  - Leucine Zippers
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Transcription Factors/metabolism
PMC - PMC44052
EDAT- 1994/06/07 00:00
MHDA- 1994/06/07 00:01
PMCR- 1994/12/07
CRDT- 1994/06/07 00:00
PHST- 1994/06/07 00:00 [pubmed]
PHST- 1994/06/07 00:01 [medline]
PHST- 1994/06/07 00:00 [entrez]
PHST- 1994/12/07 00:00 [pmc-release]
AID - 10.1073/pnas.91.12.5642 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5642-6. doi: 10.1073/pnas.91.12.5642.