PMID- 8204895
OWN - NLM
STAT- MEDLINE
DCOM- 19940712
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 83
IP  - 12
DP  - 1994 Jun 15
TI  - Oxygen transport properties in malaria-infected rodents--a comparison between 
      infected and noninfected erythrocytes.
PG  - 3746-52
AB  - This study was performed to investigate oxygen transport properties in whole 
      blood (WB) of malaria-infected rats as well as in infected erythrocytes (IE) and 
      noninfected erythrocytes (NIE) separated by density centrifugation. One week 
      after inoculation with Plasmodium berghei, mean parasitemia was 26.5% and high 
      correlations were found between parasitemia and hemoglobin concentration ([Hb]; r 
      = -.902), mean cellular Hb concentration (MCHC; r = -.712), MetHb (r = .923), and 
      base excess (r = -.922). Compared with control animals (C), the oxygen affinity 
      was lower in WB under standard (pH 7.40) and simulated "in vivo" (pH 7.00) 
      conditions (difference in P50, 5.7 and 5.1 mm Hg, respectively; 2P < .01, 2P < 
      .05). In IE Hb and 2,3-biphosphoglycerate (2,3-BPG) concentrations were decreased 
      (MCHC: IE 14.6 +/- 1.0, NIE 33.1 +/- 1.7 g/100 mL; [2,3-BPG]: IE 2.0 +/- 0.6, NIE 
      7.6 +/- 1.8 mmol/L), whereas [MetHb] and [ATP] were increased ([MetHb]: IE 19.0 
      +/- 3.7, NIE 0.7% +/- 0.8%; [ATP]: IE 33.5 +/- 2.4, NIE 6.2 +/- 1.0 mumol/g Hb). 
      At pH 7.40, half-saturation oxygen tension (P50) was reduced in IE (29.6 +/- 2.6, 
      NIE 39.2 +/- 5.4 mm Hg, 2P < .001), which correlates with lower [2,3-BPG], 
      increased MetHb content, and higher intrinsic Hb-O2 affinity. However, at pH 
      7.00, the oxygen affinity was lower in IE when compared with NIE, which was most 
      likely due to high [ATP] in IE. The resulting Bohr coefficients (BC) calculated 
      for CO2 and lactic acid were extremely high in IE and low in NIE (at 50% 
      O2-saturation BCCO2: IE -1.04 +/- 0.06, NIE -0.26 +/- 0.10, 2P < .001; BCLac: IE 
      -0.82 +/- 0.16, NIE -0.47 +/- 0.07, 2P < .001), which was caused by different 
      [2,3-BPG] and [ATP] as well as probably by structural changes of the Hb molecule. 
      The O2 capacity was 14.1 mL per 100 mL erythrocytes in IE compared with 44.4 
      mL/100 mL in NIE. On the basis of the calculated arterio-venous O2 difference 
      under "in vivo" conditions, the infected red blood cell fraction transports 30% 
      of the O2 amount delivered to the tissues by the noninfected cells (IE 8.0, NIE 
      26.9 mL/100 mL red blood cells). We conclude that the O2 transport in malaria 
      infected blood is not only affected by the degree of anemia but also by the 
      percentage of infected erythrocytes.
FAU - Schmidt, W
AU  - Schmidt W
AD  - Department of Physiology, Medical School Hannover, Germany.
FAU - Correa, R
AU  - Correa R
FAU - Boning, D
AU  - Boning D
FAU - Ehrich, J H
AU  - Ehrich JH
FAU - Kruger, C
AU  - Kruger C
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Biological Transport
MH  - Erythrocytes/*metabolism/parasitology
MH  - Female
MH  - Malaria/*blood/metabolism
MH  - Oxygen/*metabolism
MH  - Plasmodium berghei
MH  - Rats
MH  - Rats, Inbred Lew
EDAT- 1994/06/15 00:00
MHDA- 1994/06/15 00:01
CRDT- 1994/06/15 00:00
PHST- 1994/06/15 00:00 [pubmed]
PHST- 1994/06/15 00:01 [medline]
PHST- 1994/06/15 00:00 [entrez]
AID - S0006-4971(20)78873-7 [pii]
PST - ppublish
SO  - Blood. 1994 Jun 15;83(12):3746-52.