PMID- 8207834
OWN - NLM
STAT- MEDLINE
DCOM- 19940713
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 68
IP  - 7
DP  - 1994 Jul
TI  - Identification of human T-cell lymphotropic virus type I 21-base-pair 
      repeat-specific and glial cell-specific DNA-protein complexes.
PG  - 4597-608
AB  - The human T-cell lymphotropic virus type I (HTLV-I)-encoded protein, Tax, is 
      capable of trans-activating HTLV-I transcription by interacting with specific 
      sequences in the HTLV-I long terminal repeat (LTR) which comprise an inducible 
      enhancer containing three imperfect tandem repeats of a 21-bp sequence. There is 
      no evidence that purified Tax can bind to DNA in the absence of cellular factors, 
      suggesting that Tax most likely regulates transcription via interaction with 
      cellular factors. Since HTLV-I is a documented agent of adult T-cell leukemia and 
      tropical spastic paraparesis, disorders of the immune and nervous systems, 
      respectively, characterization of cellular factors of lymphoid and neuroglial 
      origin which interact with the 21-bp repeat elements is essential to 
      understanding of the mechanisms involved in basal and Tax-mediated transcription 
      in cells of immune and nervous system origin. Utilizing electrophoretic mobility 
      shift (EMS) analyses, we have detected both 21-bp repeat-specific and glial 
      cell-specific DNA-protein complexes. Several 21-bp repeat-specific DNA-protein 
      complexes were detected when nuclear extracts derived from cells of lymphoid 
      (Jurkat, SupT1, and H9), neuronal (IMR-32 and SK-N-MC), and glial (U-373 MG, 
      Hs683, and U-118) origin were used in reactions with each of the three 21-bp 
      repeat elements. In addition, a glial cell-specific DNA-protein complex was 
      detected when nuclear extracts derived from U-373 MG, Hs683, and U-118 glial cell 
      lines reacted with the promoter-distal and central 21-bp repeat elements. 
      Furthermore, EMS analyses performed with nuclear extracts derived from 
      lymphocytic and glial cell origin and a 223-bp fragment of the HTLV-I long 
      terminal repeat encompassing the three 21-bp repeat elements (designated 
      Tax-responsive elements 1 and 2, TRE-1/-2) have also resulted in the detection of 
      glial cell type-specific DNA-protein complexes. Competition EMS analyses with 
      oligonucleotides containing transcription factor binding site sequences indicate 
      the involvement of a cyclic AMP response element binding protein in the formation 
      of DNA-protein complexes which form with all three 21-bp repeat elements and the 
      glial cell-specific DNA-protein complex as well as the involvement of Sp1 or an 
      Sp1-related factor in the formation of the 21-bp repeat III-specific DNA-protein 
      complexes.
FAU - Tillmann, M
AU  - Tillmann M
AD  - Department of Microbiology and Immunology, College of Medicine, Pennsylvania 
      State University, Hershey 17033.
FAU - Wessner, R
AU  - Wessner R
FAU - Wigdahl, B
AU  - Wigdahl B
LA  - eng
GR  - CA 54559/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Gene Products, tax)
RN  - 0 (Oligodeoxyribonucleotides)
SB  - IM
MH  - Base Sequence
MH  - Cell Line
MH  - Conserved Sequence
MH  - DNA, Viral/*genetics/metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Enhancer Elements, Genetic
MH  - Gene Products, tax/metabolism
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Neuroglia/*microbiology
MH  - Oligodeoxyribonucleotides
MH  - Protein Binding
MH  - *Repetitive Sequences, Nucleic Acid
PMC - PMC236387
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
PMCR- 1994/07/01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PHST- 1994/07/01 00:00 [pmc-release]
AID - 10.1128/JVI.68.7.4597-4608.1994 [doi]
PST - ppublish
SO  - J Virol. 1994 Jul;68(7):4597-608. doi: 10.1128/JVI.68.7.4597-4608.1994.