PMID- 8207974
OWN - NLM
STAT- MEDLINE
DCOM- 19940713
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 8
IP  - 6
DP  - 1994 Jun
TI  - Fluorescence in situ hybridization improves the detection of monosomy 7 in 
      myelodysplastic syndromes.
PG  - 1012-8
AB  - We performed conventional cytogenetic (CC) and interphase fluorescence in situ 
      hybridization (FISH) analysis with an alpha satellite chromosome 7 specific DNA 
      centromeric probe (p alpha 7t1) on bone marrow material prepared for CC in 11 
      controls and 80 cases of myelodysplastic syndromes (MDS). In controls, a mean of 
      4.3 +/- 1% of the 700 cells examined showed only one FISH signal for chromosome 
      7, and the finding of > 6.3% (mean +2 standard deviations) of cells with one FISH 
      signal was considered to indicate the presence of a clone with -7. By CC, clonal 
      -7 was found in 11 patients, whereas two patients had -7 in only one mitose 
      (non-clonal -7). In eight of the 11 cases of clonal -7 by CC, interphase FISH 
      confirmed -7. In the remaining three patients, 5.1%, 6.3% and 18.4% respectively 
      of the cells had one signal. Those three patients had, in addition to -7 by CC, a 
      marker chromosome which was shown to be constituted of chromosome 7 
      pericentromeric material by FISH analysis on metaphase spreads (metaphase FISH). 
      Of the two patients with non-clonal -7 by CC, one had a -7 clone by interphase 
      FISH whereas the other patient had normal FISH results. Five of the 67 patients 
      with no -7 mitose by CC had clonal -7 by interphase FISH, with one chromosome 7 
      signal in 14.4 to 39% of the cells examined. At least three mitoses with -7 were 
      found in two of them by metaphase FISH. Three of the five patients were 
      reexamined 12 to 17 months later: CC and metaphase FISH found no -7, whereas 
      interphase FISH still showed a -7 clone. Three of the patients with clonal -7 by 
      CC and by FISH were reexamined in complete hematological remission after 
      intensive therapy. CC found no -7 and interphase FISH was normal in all three 
      patients. Our findings suggest that interphase FISH may improve the detection of 
      -7 in MDS. Conventional cytogenetics should still be performed in parallel to 
      FISH, however, because of possible false negative FISH results when a 
      pericentromeric chromosome 7 marker is present in patients with -7. Larger 
      numbers of cases with minor -7 clones, detectable by FISH only, and longer 
      follow-up in those cases will be necessary to determine the significance of this 
      finding, the evolution of this minor clone, and the outcome of the patients.
FAU - Flactif, M
AU  - Flactif M
AD  - Laboratoire de Genetique Humaine, Faculte de Medecine, Centre Hospitalier 
      Universitaire, Lille, France.
FAU - Lai, J L
AU  - Lai JL
FAU - Preudhomme, C
AU  - Preudhomme C
FAU - Fenaux, P
AU  - Fenaux P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Bone Marrow/chemistry/ultrastructure
MH  - *Chromosomes, Human, Pair 7
MH  - Follow-Up Studies
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Monosomy
MH  - Myelodysplastic Syndromes/*genetics/pathology
EDAT- 1994/06/01 00:00
MHDA- 1994/06/01 00:01
CRDT- 1994/06/01 00:00
PHST- 1994/06/01 00:00 [pubmed]
PHST- 1994/06/01 00:01 [medline]
PHST- 1994/06/01 00:00 [entrez]
PST - ppublish
SO  - Leukemia. 1994 Jun;8(6):1012-8.