PMID- 8212853 OWN - NLM STAT- MEDLINE DCOM- 19931028 LR - 20190909 IS - 0168-1702 (Print) IS - 0168-1702 (Linking) VI - 29 IP - 1 DP - 1993 Jul TI - Frequent isolation of human herpesvirus 7 from saliva. PG - 91-8 AB - We obtained isolates of human herpesvirus 7 (HHV-7) from 6 of 8 healthy adults by culturing saliva with human umbilical cord blood lymphocytes. These isolates were identified as HHV-7 on the basis of comparisons of restriction endonuclease fragment profiles and hybridization with HHV-7 strain RK DNA. The isolates could be differentiated from HHV-7 strain RK and from each other by their restriction endonuclease fragment profiles. We confirm the finding of frequent isolation of HHV-7 from saliva of healthy adults and report the first dual isolation of human herpesvirus 6 (HHV-6) and HHV-7 from a single saliva specimen. We also describe an in situ hybridization assay that can distinguish between HHV-6 and HHV-7. FAU - Black, J B AU - Black JB AD - Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333. FAU - Inoue, N AU - Inoue N FAU - Kite-Powell, K AU - Kite-Powell K FAU - Zaki, S AU - Zaki S FAU - Pellett, P E AU - Pellett PE LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Antibodies, Monoclonal) RN - 0 (Antibodies, Viral) RN - 0 (Antigens, Viral) RN - 0 (DNA, Viral) SB - IM MH - Adult MH - Antibodies, Monoclonal MH - Antibodies, Viral MH - Antigens, Viral MH - Capsid MH - Cloning, Molecular MH - Cross Reactions MH - DNA, Viral/analysis MH - Female MH - Herpesviridae Infections/*microbiology MH - Herpesvirus 7, Human/immunology/*isolation & purification MH - Humans MH - In Situ Hybridization/methods MH - Male MH - Middle Aged MH - Saliva/*microbiology EDAT- 1993/07/01 00:00 MHDA- 1993/07/01 00:01 CRDT- 1993/07/01 00:00 PHST- 1993/07/01 00:00 [pubmed] PHST- 1993/07/01 00:01 [medline] PHST- 1993/07/01 00:00 [entrez] AID - 0168-1702(93)90128-A [pii] AID - 10.1016/0168-1702(93)90128-a [doi] PST - ppublish SO - Virus Res. 1993 Jul;29(1):91-8. doi: 10.1016/0168-1702(93)90128-a.