PMID- 8214016
OWN - NLM
STAT- MEDLINE
DCOM- 19931123
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 265
IP  - 3 Pt 1
DP  - 1993 Sep
TI  - Angiotensin II type 2 receptor-modulated changes in potassium currents in 
      cultured neurons.
PG  - C607-16
AB  - We have previously shown that angiotensin II (ANG II) stimulates an increase in 
      net outward ionic current (Ino) in neurons cocultured from neonate rat 
      hypothalamus and brain stem, an effect mediated by ANG II type 2 (AT2) receptors. 
      Ino consists mainly of K+ and Ca2+ currents, and in the present study we used 
      whole cell voltage clamp procedures to define which of these currents are 
      modulated by AT2 receptors. We determined that ANG II (50-100 nM) stimulated both 
      transient K+ current (IA) and delayed-rectifier K+ current (IK) in cultured 
      neurons. The effects were mediated by AT2 receptors (blocked by 1 microM 
      PD-123177 but not by 1 microM losartan). For both IA and IK, ANG II elicited an 
      increase in maximal conductance. By contrast, ANG II altered neither 
      Ca(2+)-activated K+ current nor Ca2+ current. Our data demonstrate discrete AT2 
      receptor-mediated effects of ANG II on IA and IK in cultured neonate neurons. 
      Importantly, these data provide an electrophysiological basis for behavioral or 
      physiological effects (as yet undefined) mediated by this ANG II receptor subtype 
      in the brain.
FAU - Kang, J
AU  - Kang J
AD  - Department of Physiology, College of Medicine, University of Florida, Gainesville 
      32610.
FAU - Sumners, C
AU  - Sumners C
FAU - Posner, P
AU  - Posner P
LA  - eng
GR  - NS-19441/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Angiotensin Receptor Antagonists)
RN  - 0 (Potassium Channel Blockers)
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Tetraethylammonium Compounds)
RN  - 66-40-0 (Tetraethylammonium)
RN  - RWP5GA015D (Potassium)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Angiotensin Receptor Antagonists
MH  - Animals
MH  - Calcium/physiology
MH  - Cells, Cultured
MH  - Electric Conductivity
MH  - Neurons/metabolism/*physiology
MH  - Osmolar Concentration
MH  - Potassium/metabolism/*physiology
MH  - Potassium Channel Blockers
MH  - Rats
MH  - Receptors, Angiotensin/*physiology
MH  - Tetraethylammonium
MH  - Tetraethylammonium Compounds/pharmacology
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1152/ajpcell.1993.265.3.C607 [doi]
PST - ppublish
SO  - Am J Physiol. 1993 Sep;265(3 Pt 1):C607-16. doi: 10.1152/ajpcell.1993.265.3.C607.