PMID- 8216299
OWN - NLM
STAT- MEDLINE
DCOM- 19931122
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 196
IP  - 1
DP  - 1993 Oct 15
TI  - Growth factor mRNA profiles in unstimulated human mononuclear cells: 
      identification of genes which are constitutively and variably expressed.
PG  - 25-31
AB  - Quiescent human mononuclear cells (MNC) as studied ex vivo express highly 
      specific mRNA patterns of growth factors: We recently demonstrated that 
      unstimulated MNC constitutively express the genes for the A and B chains of 
      platelet-derived growth factor (PDGF). This expression was down-regulated by 
      dietary omega-3 fatty acids. We now report that unstimulated human MNC express 
      the genes for platelet-derived endothelial cell growth factor (PD-ECGF), 
      insulin-like growth factor (IGF-1A, -1B) and transforming growth factor-beta 1 
      (TGF-beta 1). In contrast, acidic and basic fibroblast GF (FGFs), insulin-like 
      GF-2 (IGF-2), transforming GF-alpha (TGF-alpha) and epidermal GF (EGF) were not 
      expressed in MNC, nor were alpha- and beta- receptors for PDGF. Quantitatively, 
      as measured over a period of six weeks, expression of PD-ECGF was constant, 
      whereas TGF-beta 1, IGF-1A, and IGF-1B were expressed at varying levels and all 
      independently of each other. Dietary omega-3 fatty acids had no effect on gene 
      expression. Our results also indicate that down-regulation of PDGF gene 
      expression represents a specific and possibly therapeutic effect of dietary fish 
      oil supplementation.
FAU - Jendraschak, E
AU  - Jendraschak E
AD  - Medizinische Klinik, Klinikum Innenstadt, University of Munich, FRG.
FAU - Kaminski, W E
AU  - Kaminski WE
FAU - Hessel, F
AU  - Hessel F
FAU - Kiefl, R
AU  - Kiefl R
FAU - von Schacky, C
AU  - von Schacky C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Growth Substances)
RN  - 0 (RNA, Messenger)
RN  - 0 (Somatomedins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 2.4.2.4 (Thymidine Phosphorylase)
SB  - IM
MH  - *Gene Expression Regulation
MH  - Growth Substances/*biosynthesis
MH  - Humans
MH  - Leukocytes, Mononuclear/*physiology
MH  - RNA, Messenger/*analysis
MH  - Somatomedins/biosynthesis
MH  - Thymidine Phosphorylase/biosynthesis
MH  - Transforming Growth Factor beta/biosynthesis
EDAT- 1993/10/15 00:00
MHDA- 1993/10/15 00:01
CRDT- 1993/10/15 00:00
PHST- 1993/10/15 00:00 [pubmed]
PHST- 1993/10/15 00:01 [medline]
PHST- 1993/10/15 00:00 [entrez]
AID - S0006291X83722114 [pii]
AID - 10.1006/bbrc.1993.2211 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1993 Oct 15;196(1):25-31. doi: 
      10.1006/bbrc.1993.2211.