PMID- 8217116
OWN - NLM
STAT- MEDLINE
DCOM- 19931203
LR  - 20190914
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 101
IP  - 8
DP  - 1993 Aug
TI  - Comparison of various methods and reagents for species identification of 
      Staphylococcus aureus positive or negative for the mecA gene.
PG  - 651-4
AB  - The reliability of various methods for species identification of Staphylococcus 
      aureus was evaluated. A total of 135 coagulase-positive (SA) or -negative (SS) 
      staphylococcal isolates were tested, including methicillin-resistant (MR) and 
      -susceptible (MS) strains. When the nuc gene which encodes the S. aureus 
      thermonuclease (TNase) was amplified in a multiplex PCR simultaneously with the 
      mecA gene which encodes for the MR-associated penicillin-binding protein 2a of 
      staphylococci, the nuc amplification showed full agreement with the results of 
      the coagulase test. TNase detected by an enzymatic method or as protein in a 
      sandwich ELISA identified S. aureus with nearly the same precision as the PCR. 
      The Staphylase, Monostaph and Staphaurex agglutination kits were all reliable for 
      identification of MSSA, but not for MRSA. Most of the negative MRSA strains were 
      identified by the Pastorex agglutination kit, in which reagents for fibrinogen 
      receptor and protein A detection have been supplemented with antibodies for 
      capsular polysaccharides of the serotypes 5 and 8. These results show that 
      detection of the nuc gene or its TNase product is highly reliable for 
      identification of both MRSA and MSSA strains, while various widely used 
      agglutination kits do not show the same reliability for identification of MRSA 
      strains.
FAU - Brakstad, O G
AU  - Brakstad OG
AD  - Applied Chemistry Division, SINTEF, Trondheim, Norway.
FAU - Tveten, Y
AU  - Tveten Y
FAU - Nato, F
AU  - Nato F
FAU - Fournier, J M
AU  - Fournier JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Staphylococcal Protein A)
SB  - IM
GS  - mecA
GS  - nuc
MH  - Agglutination Tests
MH  - Bacterial Proteins/analysis/genetics
MH  - DNA, Bacterial/analysis/*genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Genes, Bacterial/*genetics
MH  - Humans
MH  - Methods
MH  - Polymerase Chain Reaction
MH  - Staphylococcal Protein A/analysis/genetics
MH  - Staphylococcus aureus/*genetics/immunology/isolation & purification
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 10.1111/j.1699-0463.1993.tb00160.x [doi]
PST - ppublish
SO  - APMIS. 1993 Aug;101(8):651-4. doi: 10.1111/j.1699-0463.1993.tb00160.x.