PMID- 8218632
OWN - NLM
STAT- MEDLINE
DCOM- 19931217
LR  - 20191210
IS  - 0006-3363 (Print)
IS  - 0006-3363 (Linking)
VI  - 49
IP  - 4
DP  - 1993 Oct
TI  - Human leukocyte antigen-DR is a differentiation antigen for human granulosa 
      cells.
PG  - 705-15
AB  - We raised a murine monoclonal antibody, OG-3, which reacts with human granulosa 
      cells. Immunohistologically, OG-3 antigen was weakly expressed on the granulosa 
      cells of some growing and atretic follicles, but not on those of preovulatory 
      follicles. After ovulation, the antigen expression rapidly increased on granulosa 
      cells during corpus luteum formation. The antigen expression on granulosa/large 
      luteal cells decreased in the mid-luteal phase, but increased again in the late 
      luteal phase. In early pregnancy, OG-3 antigen expression on large luteal cells 
      increased after 7 wk of gestation. The OG-3 antigen distribution in various 
      organs resembled that of human leukocyte antigen (HLA) class II molecules. An 
      HLA-class II-positive human B cell line (AKIBA) and a murine L-cell transfectant 
      expressing HLA-DR antigen were positive for OG-3 antigen, whereas an HLA-class 
      II-negative human T-cell line and L-cell transfectants expressing HLA-DP and DQ 
      antigens were negative. The molecular mass of OG-3 antigen purified from AKIBA 
      cells was 32-35 kDa. The staining profiles in ovaries with anti-HLA-DR or 
      anti-HLA-class II antibodies were similar to that with OG-3. These results 
      indicate that OG-3 antigen is identical to HLA-DR, and that HLA-DR is a 
      differentiation antigen for human granulosa cells.
FAU - Fujiwara, H
AU  - Fujiwara H
AD  - Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, 
      Japan.
FAU - Ueda, M
AU  - Ueda M
FAU - Imai, K
AU  - Imai K
FAU - Fukuoka, M
AU  - Fukuoka M
FAU - Yasuda, K
AU  - Yasuda K
FAU - Takakura, K
AU  - Takakura K
FAU - Suginami, H
AU  - Suginami H
FAU - Kanzaki, H
AU  - Kanzaki H
FAU - Inoko, H
AU  - Inoko H
FAU - Mori, T
AU  - Mori T
AU  - et al.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Differentiation/*analysis/isolation & purification
MH  - B-Lymphocytes/immunology
MH  - Cell Line
MH  - Corpus Luteum/immunology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Granulosa Cells/*immunology
MH  - HLA-DR Antigens/*analysis/isolation & purification
MH  - Humans
MH  - L Cells/immunology
MH  - Luteal Phase
MH  - Mice
MH  - Middle Aged
MH  - Pregnancy
MH  - Tissue Distribution
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 10.1095/biolreprod49.4.705 [doi]
PST - ppublish
SO  - Biol Reprod. 1993 Oct;49(4):705-15. doi: 10.1095/biolreprod49.4.705.