PMID- 8219812
OWN - NLM
STAT- MEDLINE
DCOM- 19931209
LR  - 20191101
IS  - 0939-1983 (Print)
IS  - 0939-1983 (Linking)
VI  - 7
DP  - 1993
TI  - Molecular characterization of positive-strand RNA viruses: pestiviruses and the 
      porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 41-52
AB  - Molecular characterization has become an important tool for the analysis of 
      viruses including their classification. The manuscript focuses on the molecular 
      analysis of two members of the genus pestivirus (hog cholera virus, HCV and 
      bovine viral diarrhea virus, BVDV) and of the recently discovered porcine 
      reproductive and respiratory syndrome virus (PRRSV). The first protein encoded 
      within the single large pestivirus ORF is a nonstructural protein with 
      autoproteolytic activity. The cleavage site between the protease and the capsid 
      protein p14 has been predicted previously, but recent experimental data indicate 
      that processing occurs at a different site. The capsid protein is followed by a 
      putative internal signal sequence and three glycoproteins which are part of the 
      virion envelope. According to a new proposal for the nomenclature of the 
      structural proteins of pestiviruses they are termed C, E0, E1 and E2. The genomes 
      of BVDV pairs isolated from animals which came down with mucosal disease were 
      analyzed. The genomes from cytopathogenic (cp) BVD viruses may contain insertions 
      highly homologous to cellular sequences. In addition, cp BVDV may differ from its 
      non cytopathogenic (noncp) counterpart by mere rearrangement of viral sequences. 
      The disease PRRS, which emerged a few years ago, is caused by a single strand RNA 
      virus; the viral genome is of positive polarity and has a size of 15 kb. Data 
      concerning morphology, morphogenesis and virion composition suggested already 
      that PRRSV belongs to a group of so-called arteriviruses which comprises equine 
      arteritis virus (EAV), lactate dehydrogenase elevating virus (LDV) and simian 
      hemorrhagic fever virus (SHFV). This conclusion has now been confirmed by 
      analysis of genome organization, gene expression strategy and by comparison of 
      deduced protein sequences.
FAU - Thiel, H J
AU  - Thiel HJ
AD  - Federal Research Centre for Virus Diseases of Animals, Tubingen, Federal Republic 
      of Germany.
FAU - Meyers, G
AU  - Meyers G
FAU - Stark, R
AU  - Stark R
FAU - Tautz, N
AU  - Tautz N
FAU - Rumenapf, T
AU  - Rumenapf T
FAU - Unger, G
AU  - Unger G
FAU - Conzelmann, K K
AU  - Conzelmann KK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Austria
TA  - Arch Virol Suppl
JT  - Archives of virology. Supplementum
JID - 9214275
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Molecular Sequence Data
MH  - Pestivirus/*genetics
MH  - Swine
RF  - 33
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/978-3-7091-9300-6_4 [doi]
PST - ppublish
SO  - Arch Virol Suppl. 1993;7:41-52. doi: 10.1007/978-3-7091-9300-6_4.