PMID- 8221107
OWN - NLM
STAT- MEDLINE
DCOM- 19931213
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 623
IP  - 2
DP  - 1993 Oct 1
TI  - Functional mapping of neural sites mediating prolactin-induced hyperphagia in 
      doves.
PG  - 257-66
AB  - Microinjections of prolactin (PRL) into the ventromedial nucleus of the 
      hypothalamus (VMN) or the preoptic area (POA) have been previously shown to 
      increase food intake and body weight in ring doves. In an attempt to corroborate 
      these results and to provide a more complete map of PRL-sensitive brain sites 
      mediating the orexigenic action of PRL, a microinjection procedure was employed 
      in the present study that delivered PRL or saline vehicle in extremely small 
      volumes (10 nl/injection) to a variety of diencephalic sites in dove brain that 
      had been previously demonstrated to contain high concentrations of PRL receptors. 
      Estimates obtained from one female subject given a single 10 nl injection of 
      [125I]ovine PRL into the VMN supported the claim that such injection volumes 
      resulted in limited diffusion, as 80% of the tissue radioactivity was found 
      within a 280 mm area surrounding the injection site at 30 min after injection. 
      Food intake of cannulated male doves in the mapping study was monitored daily 
      during a 6 day baseline period, an initial 4 day treatment period, a 6-12 day 
      post-treatment recovery period, and a second 4 day treatment period. 
      Approximately half of the birds received PRL injections (50 ng/10 nl twice daily) 
      and saine vehicle injections (10 nl twice daily) during the first and second 
      treatment periods, respectively, while remaining birds received these treatments 
      in the reverse order. No significant changes in food intake across baseline, 
      vehicle, post-treatment, or PRL treatment periods were observed in birds with 
      injection sites in the lateral POA, paraventricular nucleus of the hypothalamus 
      (PVN), or the medial-basal hypothalamic region between the tuberal hypothalamus 
      (TU) and VMN. In contrast, injections of PRL into the VMN area, medial POA, or TU 
      resulted in average daily food intake values that significantly exceeded those 
      recorded during other periods. The most robust feeding response was seen in the 
      VMN group, where PRL injections resulted in a 58% increase in food intake over 
      that recorded during injection of vehicle. This increase was significantly 
      greater than that observed following PRL injections into the mPOA (26%) or the TU 
      (32%). These findings suggest that the VMN may be a primary site of PRL action in 
      promoting hyperphagia in this species, although PRL effects at other diencephalic 
      loci, such as the mPOA and TU, may also contribute to the orexigenic action of 
      this hormone.
FAU - Hnasko, R M
AU  - Hnasko RM
AD  - Department of Biological Sciences, University of Wisconsin at Milwaukee 53201.
FAU - Buntin, J D
AU  - Buntin JD
LA  - eng
GR  - NIMH MH41447/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 9002-62-4 (Prolactin)
SB  - IM
MH  - Animals
MH  - Birds/*physiology
MH  - *Brain Mapping
MH  - Diencephalon/*physiology
MH  - Eating/drug effects/physiology
MH  - Feeding Behavior/drug effects/physiology
MH  - Female
MH  - Hyperphagia/*chemically induced
MH  - Hypothalamic Area, Lateral/physiology
MH  - Male
MH  - Preoptic Area/physiology
MH  - *Prolactin
MH  - Ventromedial Hypothalamic Nucleus/physiology
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0006-8993(93)91436-V [pii]
AID - 10.1016/0006-8993(93)91436-v [doi]
PST - ppublish
SO  - Brain Res. 1993 Oct 1;623(2):257-66. doi: 10.1016/0006-8993(93)91436-v.