PMID- 8221608
OWN - NLM
STAT- MEDLINE
DCOM- 19931207
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 70
IP  - 1
DP  - 1993 Oct 1
TI  - In situ hybridization: a simple and sensitive method for detection of trisomy 12 
      in chronic lymphocytic leukemia.
PG  - 21-4
AB  - Chromosome aberrations are detected in only 50% of patients with chronic 
      lymphocytic leukemia (CLL), owing usually to the low mitotic rate exhibited by 
      the neoplastic lymphocytes. Fluorescence in situ hybridization (FISH) is a simple 
      method for identifying numerical abnormalities of the target chromosome in 
      interphase nuclei. Therefore, we used the FISH procedure with chromosome 
      12-specific a-satellite probe to evaluate 19 patients with CLL. Trisomy 12 was 
      detected in interphase cells of 12 patients (63%). Cytogenetic analysis, 
      performed in nine patients, yielded trisomy 12 in four (44%). FISH detected three 
      patients with trisomy 12 in whom conventional cytogenetic method yielded a normal 
      karyotype. FISH is a simple, reliable, and sensitive method for detection of 
      trisomy 12 in patients with CLL.
FAU - Lalkin, A
AU  - Lalkin A
AD  - Department of Medicine, Meir Hospital, Kfar Saba, Israel.
FAU - Lishner, M
AU  - Lishner M
FAU - Gaber, E
AU  - Gaber E
FAU - Manor, Y
AU  - Manor Y
FAU - Fejgin, M
AU  - Fejgin M
FAU - Ravid, M
AU  - Ravid M
FAU - Amiel, A
AU  - Amiel A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Aged
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*diagnosis/pathology
MH  - Male
MH  - Middle Aged
MH  - Trisomy/*diagnosis
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0165-4608(93)90126-7 [pii]
AID - 10.1016/0165-4608(93)90126-7 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 Oct 1;70(1):21-4. doi: 10.1016/0165-4608(93)90126-7.