PMID- 8222623
OWN - NLM
STAT- MEDLINE
DCOM- 19931208
LR  - 20190721
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 29
IP  - 3
DP  - 1993 Sep
TI  - Influence of irritants on lymph node cell proliferation and the detection of 
      contact sensitivity to metal salts in the murine local lymph node assay.
PG  - 128-32
AB  - Dimethyl sulfoxide (DMSO) and sodium lauryl sulfate (SLS) are known to cause 
      irritation of the skin, and to enhance the penetration of chemicals into the 
      epidermis. In the present study, the lymph node cell (LNC) proliferative response 
      following exposure to irritants, such as SLS and DMSO, was examined in the murine 
      local lymph node assay (LLNA). Exposure to DMSO or SLS aqueous solution induced a 
      small increase in lymph node cell proliferation compared with aqueous solution 
      alone. Exposure to SLS in DMSO caused a significant increase in LNC 
      proliferation. Further, the effect of addition of the irritants in a vehicle on 
      the detection of contact sensitivity to metal allergens was examined. Application 
      of potassium dichromate and nickel sulfate in DMSO or SLS aqueous solution caused 
      increases in LNC proliferation. Exposure to metal allergen with SLS in DMSO also 
      induced a significant LNC proliferative response, but did not induce a 
      significant increase in stimulation index (increase in 3H-thymidine incorporation 
      relative to vehicle-treated control group). This was because of increased 
      3H-thymidine incorporation following exposure to SLS-DMSO in the control group. 
      These results suggest that irritants enhance the LNC proliferative responses to 
      metal allergens. The use of SLS in aqueous solution is effective for the 
      detection of sensitivity to water-soluble allergens, such as metal allergens, in 
      the LLNA, as well as the use of DMSO as an application vehicle.
FAU - Ikarashi, Y
AU  - Ikarashi Y
AD  - National Institute of Hygienic Sciences, Tokyo, Japan.
FAU - Tsukamoto, Y
AU  - Tsukamoto Y
FAU - Tsuchiya, T
AU  - Tsuchiya T
FAU - Nakamura, A
AU  - Nakamura A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Irritants)
RN  - 0 (Metals)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - 4FLT4T3WUN (nickel sulfate)
RN  - 7OV03QG267 (Nickel)
RN  - T4423S18FM (Potassium Dichromate)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Dimethyl Sulfoxide/toxicity
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Irritants/*toxicity
MH  - Lymphocyte Activation/*drug effects/immunology
MH  - Metals/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nickel/immunology
MH  - Potassium Dichromate/immunology
MH  - Skin Absorption/drug effects/immunology
MH  - Sodium Dodecyl Sulfate/toxicity
MH  - T-Lymphocytes/*drug effects/immunology
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1111/j.1600-0536.1993.tb03509.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 1993 Sep;29(3):128-32. doi: 
      10.1111/j.1600-0536.1993.tb03509.x.