PMID- 8222753
OWN - NLM
STAT- MEDLINE
DCOM- 19931215
LR  - 20240109
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 65
IP  - 3
DP  - 1994
TI  - Increased FISH efficiency using APC probes generated by direct incorporation of 
      labeled nucleotides by PCR.
PG  - 169-71
AB  - Probes of various sizes from the adenomatous polyposis coli gene (APC) were 
      directly biotinylated by polymerase chain reaction (PCR) from genomic DNA. PCR 
      labeling gave high efficiency in detection of fluorescence in situ hybridization 
      (FISH) signals. Probes as small as 250 base pairs could be visualized through a 
      fluorescence microscope without any image processing.
FAU - Richard, F
AU  - Richard F
AD  - CNRS URA 620, Institut Curie, Section de Biologie, Paris, France.
FAU - Vogt, N
AU  - Vogt N
FAU - Muleris, M
AU  - Muleris M
FAU - Malfoy, B
AU  - Malfoy B
FAU - Dutrillaux, B
AU  - Dutrillaux B
LA  - eng
SI  - GENBANK/M73548
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
RN  - 6SO6U10H04 (Biotin)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
GS  - APC
MH  - Adenomatous Polyposis Coli/*genetics
MH  - Base Sequence
MH  - Biotin
MH  - DNA Primers/chemistry
MH  - DNA Probes/*chemistry
MH  - Digoxigenin
MH  - Exons
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1159/000133624 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1994;65(3):169-71. doi: 10.1159/000133624.