PMID- 8223477
OWN - NLM
STAT- MEDLINE
DCOM- 19931221
LR  - 20220511
IS  - 0261-4189 (Print)
IS  - 1460-2075 (Electronic)
IS  - 0261-4189 (Linking)
VI  - 12
IP  - 12
DP  - 1993 Dec
TI  - Synthesis of reinitiated transcripts by mammalian RNA polymerase II is controlled 
      by elongation factor SII.
PG  - 4677-84
AB  - Previous studies have revealed that the in vitro synthesis of reinitiated 
      transcripts by RNA polymerase II requires an additional activity, designated 
      reinitiation transcription factor (RTF), which is distinct from all of the 
      general class II initiation factors. While further characterizing this activity, 
      it was found that RTF displays properties indistinguishable from those of the RNA 
      polymerase II elongation factor SII. In addition, Western blot analysis using 
      SII-specific antibodies revealed that human SII is a major component in purified 
      RTF preparations. The functional equivalence of the two proteins was established 
      using recombinant SII, which proved fully capable of substituting for RTF in the 
      reinitiation assay. In these reconstituted reactions, transcription complexes 
      resulting from reinitiation events required SII to proceed through a 400 bp 
      G-free cassette, while complexes resulting from the first round of initiations 
      were SII-independent. Reinitiations can take place in the absence of SII; 
      however, addition of the elongation factor is essential for full extension of the 
      reinitiated transcripts. These results suggest that events taking place at the 
      promoter (e.g. first-round initiations versus reinitiations) can create marked 
      differences in the properties of RNA polymerase II elongation complexes.
FAU - Szentirmay, M N
AU  - Szentirmay MN
AD  - Department of Molecular Genetics, University of Texas M.D. Anderson Cancer 
      Center, Houston, TX 77030.
FAU - Sawadogo, M
AU  - Sawadogo M
LA  - eng
GR  - CA-09299/CA/NCI NIH HHS/United States
GR  - GM-38212/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - EMBO J
JT  - The EMBO journal
JID - 8208664
RN  - 0 (Transcription Factors)
RN  - 0 (Transcription Factors, General)
RN  - 0 (Transcriptional Elongation Factors)
RN  - 0 (transcription factor S-II)
RN  - EC 2.7.7.- (RNA Polymerase II)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - RNA Polymerase II/*metabolism
MH  - Transcription Factors/*metabolism
MH  - *Transcription Factors, General
MH  - *Transcription, Genetic
MH  - *Transcriptional Elongation Factors
PMC - PMC413907
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
PMCR- 1994/12/01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PHST- 1994/12/01 00:00 [pmc-release]
AID - 10.1002/j.1460-2075.1993.tb06156.x [doi]
PST - ppublish
SO  - EMBO J. 1993 Dec;12(12):4677-84. doi: 10.1002/j.1460-2075.1993.tb06156.x.