PMID- 8223707
OWN - NLM
STAT- MEDLINE
DCOM- 19931206
LR  - 20091119
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 61
IP  - 1
DP  - 1993 Jun
TI  - Circular ruffle formation and closure lead to macropinocytosis in hepatocyte 
      growth factor/scatter factor-treated cells.
PG  - 44-53
AB  - Treatment with hepatocyte growth factor/scatter factor (HGF/SF) rapidly induced 
      the formation of conspicuous circular ruffles on the apical surfaces of two 
      kidney cell lines, MDCK and PtK2. The ruffles were found to contain significant 
      amounts of F-actin and myosin as judged by immunofluorescence microscopy. 
      Time-lapse photomicroscopy demonstrated that the ruffles constrict, closing over, 
      and were followed by the formation of phase bright structures. That these 
      structures were macropinocytotic vesicles was confirmed using fluorescein 
      isothiocyanate (FITC)-dextran as a marker for fluid uptake. It is hypothesized 
      that the constriction of the ruffles followed by membrane fusion causes the 
      vesicles to form. Treatment with suramin blocked both circular ruffle formation 
      and scattering, suggesting that ligand binding was the causal agent for ruffle 
      formation. The drugs amiloride and SITS 
      (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid) also completely 
      inhibited ruffle formation, suggesting that ion transport was an early 
      consequence of HGF/SF binding and that these transport effects had a major role 
      in the cytoskeletal changes leading to circular ruffle formation. The appearance 
      of macropinocytotic vesicles was also blocked by amiloride treatment. 
      Surprisingly though, subsequent scattering was not blocked by amiloride 
      treatment, although suramin and SITS both entirely inhibited scattering.
FAU - Dowrick, P
AU  - Dowrick P
AD  - School of Biological Sciences, University of East Anglia, Norwich/United Kingdom.
FAU - Kenworthy, P
AU  - Kenworthy P
FAU - McCann, B
AU  - McCann B
FAU - Warn, R
AU  - Warn R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Actins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.6.4.1 (Myosins)
SB  - IM
MH  - 3T3 Cells
MH  - Actins/drug effects
MH  - Animals
MH  - Cell Line
MH  - Dogs
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Kidney/*drug effects/ultrastructure
MH  - Mice
MH  - Microscopy, Electron, Scanning
MH  - Microscopy, Phase-Contrast
MH  - Myosins/drug effects
MH  - Pinocytosis/*drug effects
EDAT- 1993/06/01 00:00
MHDA- 1993/06/01 00:01
CRDT- 1993/06/01 00:00
PHST- 1993/06/01 00:00 [pubmed]
PHST- 1993/06/01 00:01 [medline]
PHST- 1993/06/01 00:00 [entrez]
PST - ppublish
SO  - Eur J Cell Biol. 1993 Jun;61(1):44-53.