PMID- 8224167
OWN - NLM
STAT- MEDLINE
DCOM- 19931126
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 333
IP  - 1-2
DP  - 1993 Oct 25
TI  - Association constants of anti-hapten monoclonal IgG1 with mouse Fc gamma RII in 
      the presence and absence of hapten.
PG  - 35-8
AB  - The binding of five different mouse monoclonal anti-dinitrophenyl (DNP) IgGl 
      monomers, in the presence and absence of DNP-glycine, to Fc gamma RII on the 
      mouse macrophage-like cell line J774 has been investigated. Membrane association 
      constants were estimated using a competitive radioimmunoassay in which the 
      ability of the IgG1 monomers to block the binding of 125I-labelled Fabs of the 
      anti-Fc gamma RII monoclonal antibody 2.4G2 was monitored. The IgG1-Fc gamma RII 
      association constants ranged from (8 +/- 1) x 10(4) M-1 to (6 +/- 2) x 10(5) M-1. 
      No large differences in the IgG1-Fc gamma RII association constants were measured 
      for different IgG1 antibodies or for any single IgG1 antibody in the presence and 
      absence of DNP-glycine.
FAU - Sumner, M T
AU  - Sumner MT
AD  - Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290.
LA  - eng
GR  - GM-37145/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Haptens)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Binding Sites, Antibody
MH  - Cell Line
MH  - Haptens/*immunology
MH  - Hybridomas
MH  - Immunoglobulin G/*immunology
MH  - Mice
MH  - Rats
MH  - Receptors, IgG/*immunology
EDAT- 1993/10/25 00:00
MHDA- 1993/10/25 00:01
CRDT- 1993/10/25 00:00
PHST- 1993/10/25 00:00 [pubmed]
PHST- 1993/10/25 00:01 [medline]
PHST- 1993/10/25 00:00 [entrez]
AID - 001457939380370A [pii]
AID - 10.1016/0014-5793(93)80370-a [doi]
PST - ppublish
SO  - FEBS Lett. 1993 Oct 25;333(1-2):35-8. doi: 10.1016/0014-5793(93)80370-a.