PMID- 8225024
OWN - NLM
STAT- MEDLINE
DCOM- 19931217
LR  - 20190825
IS  - 0891-5849 (Print)
IS  - 0891-5849 (Linking)
VI  - 15
IP  - 4
DP  - 1993 Oct
TI  - Spin trapping of superoxide radicals following stimulation of neutrophils with 
      fMLP is temperature dependent.
PG  - 425-33
AB  - Oxygen radical formation by human neutrophils stimulated with a chemotactic 
      peptide, formyl-methionyl-leucyl-phenylalanine (fMLP), was studied through the 
      use of spin trapping and superoxide dismutase-inhibitable reduction of oxidized 
      cytochrome c. Both methods provided comparable data on temperature-dependent 
      kinetics of superoxide radical formation, but hydroxyl radicals were also 
      detected in spin-trapping experiments. When superoxide generation was monitored 
      at 37 degrees C, the respiratory burst lasted only a few minutes. If the 
      neutrophils were stimulated at 37 degrees C, but superoxide measurements were 
      done at room temperature, the respiratory burst was again transient. However, 
      neutrophils persistently generated superoxide when both stimulation and 
      subsequent measurements were performed at room temperature. In the presence of 
      the actin polymerization inhibitor, cytochalasin B, superoxide generation was 
      persistent, even when measurements were conducted at 37 degrees C. A possible 
      explanation for these observations is that the fMLP receptor complexes quickly 
      aggregate and are internalized at physiological temperature, but not at room 
      temperature. Very little superoxide was formed if cells were kept at a 
      temperature of 4 degrees C for 1 h prior to fMLP addition, which is consistent 
      with decreased expression of the fMLP receptor at cold temperatures.
FAU - Tanigawa, T
AU  - Tanigawa T
AD  - National Biomedical Center for Spin Trapping and Free Radicals, Oklahoma Medical 
      Research Foundation, Oklahoma City.
FAU - Kotake, Y
AU  - Kotake Y
FAU - Reinke, L A
AU  - Reinke LA
LA  - eng
GR  - RR0 5517-01A1/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Cytochrome c Group)
RN  - 0 (Free Radicals)
RN  - 11062-77-4 (Superoxides)
RN  - 3CHI920QS7 (Cytochalasin B)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
SB  - IM
MH  - Cytochalasin B/pharmacology
MH  - Cytochrome c Group/metabolism
MH  - *Electron Spin Resonance Spectroscopy
MH  - Free Radicals
MH  - Humans
MH  - N-Formylmethionine Leucyl-Phenylalanine/*pharmacology
MH  - Neutrophils/*metabolism
MH  - Oxidation-Reduction
MH  - Superoxides/*blood
MH  - Temperature
EDAT- 1993/10/01 00:00
MHDA- 1993/10/01 00:01
CRDT- 1993/10/01 00:00
PHST- 1993/10/01 00:00 [pubmed]
PHST- 1993/10/01 00:01 [medline]
PHST- 1993/10/01 00:00 [entrez]
AID - 0891-5849(93)90042-S [pii]
AID - 10.1016/0891-5849(93)90042-s [doi]
PST - ppublish
SO  - Free Radic Biol Med. 1993 Oct;15(4):425-33. doi: 10.1016/0891-5849(93)90042-s.