PMID- 8225495
OWN - NLM
STAT- MEDLINE
DCOM- 19931206
LR  - 20071115
IS  - 0300-5038 (Print)
IS  - 0300-5038 (Linking)
IP  - 124
DP  - 1993
TI  - Measurement of oxidative base damage to DNA by using HPLC-32P-postlabelling and 
      GC/MS-selective ion monitoring assays.
PG  - 271-6
AB  - A 32P-postlabelling assay has been developed for singling out specific oxidized 
      base lesions. Emphasis was placed on the quantitative aspect and the accuracy of 
      the assay, which require the use of calibration curves and microreactions, 
      respectively. The method was successfully applied to the detection and the 
      measurement of adenine N1-oxide and 5-hydroxymethyluracil in cells exposed to 
      agents inducing oxidative stress including H2O2 and UV-A radiation. The 
      sensitivity of the assay allows the detection of one lesion in 10(6) normal bases 
      in 1 microgram of DNA. The GC/MS method when coupled to the selective ion 
      monitoring (SIM) technique is about twenty times less sensitive, even for 
      suitable substrates such as 5-hydroxymethyluracil and 5-hydroxyuracil, than the 
      32P-postlabelling assay. However, the former assay is much easier to apply, even 
      though a derivatization step is necessary, and provides unambiguous structural 
      information on the compound to be measured. Accurate quantitative measurements 
      can be obtained when stable, isotopically labelled standards are available.
FAU - Cadet, J
AU  - Cadet J
AD  - Laboratoire des Lesions des Acides Nucleiques, SESAM/DRFMC, Centre d'Etudes 
      Nucleaires, Grenoble, France.
FAU - Incardona, M F
AU  - Incardona MF
FAU - Odin, F
AU  - Odin F
FAU - Molko, D
AU  - Molko D
FAU - Mouret, J F
AU  - Mouret JF
FAU - Polverelli, M
AU  - Polverelli M
FAU - Faure, H
AU  - Faure H
FAU - Ducros, V
AU  - Ducros V
FAU - Tripier, M
AU  - Tripier M
FAU - Favier, A
AU  - Favier A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - France
TA  - IARC Sci Publ
JT  - IARC scientific publications
JID - 8009542
RN  - 0 (Phosphorus Radioisotopes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods/statistics & numerical data
MH  - DNA/*analysis/chemistry/drug effects
MH  - *DNA Damage
MH  - Environmental Monitoring/methods/statistics & numerical data
MH  - Evaluation Studies as Topic
MH  - Gas Chromatography-Mass Spectrometry/methods/standards/statistics & numerical 
      data
MH  - Oxidation-Reduction
MH  - *Phosphorus Radioisotopes
MH  - Reference Standards
MH  - Sensitivity and Specificity
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
PST - ppublish
SO  - IARC Sci Publ. 1993;(124):271-6.