PMID- 8225596
OWN - NLM
STAT- MEDLINE
DCOM- 19931222
LR  - 20220321
IS  - 0019-9567 (Print)
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Linking)
VI  - 61
IP  - 12
DP  - 1993 Dec
TI  - Expression of monocyte chemoattractant protein 1 (MCP-1) in adult periodontal 
      disease: increased monocyte chemotactic activity in crevicular fluids and 
      induction of MCP-1 expression in gingival tissues.
PG  - 5219-24
AB  - The present study shows that monocyte chemotactic activity in crevicular fluids 
      increases with severity of the disease and that a monocyte chemoattractant, 
      monocyte chemoattractant protein 1 (MCP-1), is expressed as the predominant 
      cytokine of gingival tissues and their fibroblasts treated with Porphyromonas 
      (Bacteroides) gingivalis lipopolysaccharide (P-LPS). High monocyte chemotactic 
      activity in the crevicular fluids was neutralized significantly by antiserum 
      specific for the JE/MCP-1 protein. Marked expression of the MCP-1 gene was 
      observed in the gingival tissues of all adult periodontal patients tested, but 
      not in those of healthy subjects. Monocyte chemotactic activity was observed in 
      culture supernatants of human normal gingival tissues treated with P-LPS, and the 
      chemotactic activity increased in a dose-related manner. Expression of MCP-1 in 
      P-LPS-treated human gingival fibroblasts was further examined. P-LPS induced the 
      MCP-1 gene expression in a dose- and treatment time-dependent manner. The MCP-1 
      gene product in the culture supernatant was detected as two forms with molecular 
      masses of 11,000 and 15,000 Da by immunoprecipitation with the specific 
      antiserum. The MCP-1 gene expression was induced in the fibroblasts treated with 
      interleukin-1 beta and tumor necrosis factor alpha, but not with interleukin-6. 
      These results suggest that gingival fibroblasts can participate in monocyte 
      recruitment in gingival tissues of adult periodontal patients via the MCP-1 gene 
      product and that MCP-1 plays an important role in the inflammatory reaction in 
      the disease.
FAU - Hanazawa, S
AU  - Hanazawa S
AD  - Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, 
      Japan.
FAU - Kawata, Y
AU  - Kawata Y
FAU - Takeshita, A
AU  - Takeshita A
FAU - Kumada, H
AU  - Kumada H
FAU - Okithu, M
AU  - Okithu M
FAU - Tanaka, S
AU  - Tanaka S
FAU - Yamamoto, Y
AU  - Yamamoto Y
FAU - Masuda, T
AU  - Masuda T
FAU - Umemoto, T
AU  - Umemoto T
FAU - Kitano, S
AU  - Kitano S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Antibodies)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
GS  - MCP-1
MH  - Adult
MH  - Antibodies
MH  - Chemokine CCL2
MH  - Chemotactic Factors/genetics/*metabolism
MH  - Chemotaxis, Leukocyte
MH  - Cytokines/pharmacology
MH  - Gene Expression/drug effects
MH  - Gingiva/drug effects/*immunology/pathology
MH  - Gingival Crevicular Fluid/immunology
MH  - Humans
MH  - In Vitro Techniques
MH  - Lipopolysaccharides/pharmacology
MH  - Monocytes/immunology/pathology
MH  - Neutralization Tests
MH  - Periodontal Diseases/genetics/*immunology/pathology
MH  - Porphyromonas gingivalis/immunology
PMC - PMC281304
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
PMCR- 1993/12/01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PHST- 1993/12/01 00:00 [pmc-release]
AID - 10.1128/iai.61.12.5219-5224.1993 [doi]
PST - ppublish
SO  - Infect Immun. 1993 Dec;61(12):5219-24. doi: 10.1128/iai.61.12.5219-5224.1993.