PMID- 8225890
OWN - NLM
STAT- MEDLINE
DCOM- 19931215
LR  - 20151119
IS  - 0020-9996 (Print)
IS  - 0020-9996 (Linking)
VI  - 28
IP  - 9
DP  - 1993 Sep
TI  - Cerebral embolization in rats induced by red blood cells treated with hypertonic 
      X-ray contrast medium.
PG  - 831-7
AB  - RATIONALE AND OBJECTIVES: Hypertonic X-ray contrast media induce morphologic 
      changes in red blood cells (RBCs) and reduce their deformability when measured in 
      vitro. This study investigated whether RBCs treated with hypertonic contrast 
      media could induce circulatory as well as metabolic disturbances in vivo. 
      METHODS: Autologous blood was mixed with an equal volume of meglumine diatrizoate 
      (306 mgI/mL); 50 microliters of this mixture was infused into the left internal 
      carotid artery of rats 2 minutes after mixing. Five minutes after infusion, the 
      cerebral blood flow was determined using iodo[14C]antipyrine. 31P-magnetic 
      resonance spectroscopy (MRS), 1H-MRS and 1H-magnetic resonance imaging (MRI) of 
      the brain were performed before and after infusion of the mixture to study 
      changes in energy metabolites and the integrity of the blood-brain barrier (BBB), 
      as well as the development of edema. RESULTS: The blood flow decreased by 70% to 
      80% in the left cerebral cortex and caudate-putamen. 31P-MRS and 1H-MRS 
      demonstrated derangement of oxidative phosphorylation. 1H-MRI demonstrated 
      instant destruction of the BBB and gradual progress of edema in the left 
      hemisphere. CONCLUSIONS: These results indicate that intracarotid infusion of 
      RBCs treated with hypertonic contrast medium can induce embolization and 
      subsequent ischemic damage to the brain.
FAU - Miyazawa, T
AU  - Miyazawa T
AD  - Nihon Schering K.K., Research Department, Osaka, Japan.
FAU - Murayama, C
AU  - Murayama C
FAU - Yamanaka, T
AU  - Yamanaka T
FAU - Fritz-Zieroth, B
AU  - Fritz-Zieroth B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Invest Radiol
JT  - Investigative radiology
JID - 0045377
RN  - 0 (Hypertonic Solutions)
RN  - 0 (Lactates)
RN  - 0 (Phosphates)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3X9MR4N98U (Diatrizoate Meglumine)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Blood-Brain Barrier
MH  - Brain/metabolism
MH  - Cerebrovascular Circulation
MH  - Diatrizoate Meglumine/*toxicity
MH  - Erythrocytes/*drug effects/physiology
MH  - Hydrogen-Ion Concentration
MH  - Hypertonic Solutions
MH  - Intracranial Embolism and Thrombosis/blood/*chemically 
      induced/metabolism/physiopathology
MH  - Lactates/metabolism
MH  - Lactic Acid
MH  - Magnetic Resonance Spectroscopy
MH  - Male
MH  - Phosphates/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
PST - ppublish
SO  - Invest Radiol. 1993 Sep;28(9):831-7.