PMID- 8228354
OWN - NLM
STAT- MEDLINE
DCOM- 19931129
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 168
IP  - 5
DP  - 1993 Nov
TI  - Interleukin-8 production by human peritoneal mesothelial cells in response to 
      tumor necrosis factor-alpha, interleukin-1, and medium conditioned by macrophages 
      cocultured with Staphylococcus epidermidis.
PG  - 1202-10
AB  - Patients treated with continuous ambulatory peritoneal dialysis (CAPD) may suffer 
      from recurrent peritonitis episodes caused by Staphylococcus epidermidis. Early 
      recruitment of granulocytes from the peripheral blood is important for the 
      peritoneal antibacterial defense of CAPD patients. In this study, human 
      peritoneal mesothelial cells were shown to produce high levels of interleukin-8 
      (IL-8) in response to IL-1 beta and tumor necrosis factor-alpha (TNF alpha) but 
      not lipopolysaccharide or S. epidermidis. Coculture of peritoneal macrophages 
      with S. epidermidis induced high levels of IL-1 alpha, IL-1 beta, and TNF alpha 
      in 24-h-conditioned medium. Preincubation of this medium with anti-TNF alpha, 
      anti-IL-1 alpha, or anti-IL-1 beta partially blocked stimulation of IL-8 
      production by mesothelial cells. Added together, these antibodies abolished IL-8 
      production to a level just above background. Migration of granulocytes to the 
      stimulated mesothelial cell-conditioned medium could be totally blocked with 
      rabbit polyclonal anti-IL-8 antibody. Thus, mesothelial cells are important for 
      the recruitment of granulocytes into the peritoneal cavity.
FAU - Betjes, M G
AU  - Betjes MG
AD  - Dept. of Cell Biology, Medical Faculty, Vrije Universiteit, Amsterdam, 
      Netherlands.
FAU - Tuk, C W
AU  - Tuk CW
FAU - Struijk, D G
AU  - Struijk DG
FAU - Krediet, R T
AU  - Krediet RT
FAU - Arisz, L
AU  - Arisz L
FAU - Hart, M
AU  - Hart M
FAU - Beelen, R H
AU  - Beelen RH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cells, Cultured
MH  - Chemotaxis, Leukocyte
MH  - Culture Media, Conditioned/chemistry/pharmacology
MH  - Cytokines/analysis
MH  - Humans
MH  - Interleukin-1/analysis/*pharmacology
MH  - Interleukin-8/*biosynthesis
MH  - Macrophages, Peritoneal/metabolism
MH  - Peritoneum/cytology/*metabolism
MH  - Phagocytosis
MH  - Staphylococcus epidermidis/*metabolism
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/analysis/*pharmacology
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 10.1093/infdis/168.5.1202 [doi]
PST - ppublish
SO  - J Infect Dis. 1993 Nov;168(5):1202-10. doi: 10.1093/infdis/168.5.1202.