PMID- 8228574
OWN - NLM
STAT- MEDLINE
DCOM- 19931214
LR  - 20071114
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 122
IP  - 5
DP  - 1993 Nov
TI  - Flow cytometric analysis of residual white blood cell concentration and platelet 
      activation antigens in double filtered platelet concentrates.
PG  - 557-66
AB  - Reduction of contaminating leukocytes in platelet products by filtration has been 
      shown to decrease the incidence of human leukocyte antigen (HLA) 
      alloimmunization. Nonetheless, prevention is not complete when using current 
      techniques, and a significant number of patients continue to exhibit clinical 
      refractoriness and to produce alloantibodies. Interest in preventing HLA 
      alloimmunization and other complications of white blood cell (WBC) contamination 
      of transfused cellular products has resulted in ongoing efforts to increase the 
      efficiency of leukodepletion filters. As the efficiency of these filters 
      increases, more accurate and precise methods for counting extremely low numbers 
      of WBCs must be instituted to ensure quality control. We have validated a simple, 
      rapid flow cytometric assay for quantitating low numbers of WBCs in platelet 
      products. The assay is sensitive to a lower limit of 0.1 WBC/microliter without 
      concentration of the platelet product sample and has an excellent correlation (R2 
      = 1.00) between calculated and expected WBC concentration over a range of 0.1 to 
      100.0 WBC/microliter. (R2 values over the concentration ranges of 0.1 to 1.0 
      WBC/microliter and 1.0 to 10.0 WBC/microliter were 0.988 and 0.996, 
      respectively.) The intraassay coefficients of variation at WBC concentrations of 
      50.4/microliter, 0.9/microliter, and 0.1/microliter were 4%, 8%, and 18%, 
      respectively. The flow cytometric counting technique was applied, in concert with 
      a Nageotte chamber manual counting method, to the enumeration of residual WBCs in 
      20 apheresis and random donor platelet concentrates filtered through two 
      leukodepletion filters sterile docked in series. A greater than four log10 WBC 
      reduction capability was demonstrated when utilizing this double filtration 
      procedure, and its clinical applicability is underscored by data that showed no 
      statistically significant change in expression of activation-specific platelet 
      antigens before versus after filtration.
FAU - Lombardo, J F
AU  - Lombardo JF
AD  - Department of Pediatrics, University of Colorado School of Medicine, Denver.
FAU - Cusack, N A
AU  - Cusack NA
FAU - Rajagopalan, C
AU  - Rajagopalan C
FAU - Sangaline, R J
AU  - Sangaline RJ
FAU - Ambruso, D R
AU  - Ambruso DR
LA  - eng
GR  - K07-HL02036/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Antibodies, Monoclonal/analysis/immunology
MH  - Antibody Specificity
MH  - Blood Component Removal
MH  - Blood Platelets/*cytology/*immunology
MH  - Flow Cytometry
MH  - HLA Antigens/*analysis
MH  - Humans
MH  - *Leukocyte Count
MH  - Leukocytes/*cytology
MH  - Platelet Count
MH  - Platelet Membrane Glycoproteins/immunology
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 0022-2143(93)90015-Q [pii]
PST - ppublish
SO  - J Lab Clin Med. 1993 Nov;122(5):557-66.