PMID- 8230454
OWN - NLM
STAT- MEDLINE
DCOM- 19931221
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 67
IP  - 12
DP  - 1993 Dec
TI  - Transcriptional activation of the human T-lymphotropic virus type I long terminal 
      repeat by functional interaction of Tax1 and Ets1.
PG  - 7307-16
AB  - Transcription regulation of the oncogenic retrovirus human T-lymphotropic virus 
      type I (HTLV-I) involves the composite activity of both viral and cellular 
      transcription factors. The HTLV-I transforming protein, Tax1, modulates the 
      activity of several cellular transcription factors, upregulating the level of 
      viral gene expression. In addition, cellular transcription factors, such as Ets1, 
      independently bind to the viral long terminal repeat in a sequence-specific 
      manner and activate transcription. It was of interest to analyze the possible 
      interaction of Tax1 and Ets1 in viral gene regulation. We now report that Tax1 
      and Ets1 increase expression from the HTLV-I promoter in a cooperative manner. 
      The level of expression was increased 5- to 10-fold above the combined individual 
      effect of Tax1 and Ets1. S1 nuclease analysis demonstrated that the cooperative 
      effect was due to an increase in the levels of steady-state RNA. The functional 
      interaction between Tax1 and Ets1 required the presence of the Tax1-responsive 
      21-bp repeat element TRE-1 and the Ets1-responsive element ERR-1. These results 
      suggested the possible interaction of Ets1 with transcriptional regulatory 
      proteins that bind to the 21-bp repeats. This interaction is demonstrated by 
      decreased electrophoretic mobility of specific 21-bp repeat gel shift complexes 
      in the presence of Ets1. Furthermore, interaction of Ets1 with the 21-bp 
      repeat-binding proteins enhances the relative efficiency of binding to the DNA. 
      This cooperative interaction between Ets1 and proteins which bind to the 
      Tax1-responsive 21-bp repeats suggests a possible role for Ets1 in the regulation 
      of viral gene expression.
FAU - Gitlin, S D
AU  - Gitlin SD
AD  - Laboratory of Molecular Virology, National Cancer Institute, Bethesda, Maryland 
      20892.
FAU - Dittmer, J
AU  - Dittmer J
FAU - Shin, R C
AU  - Shin RC
FAU - Brady, J N
AU  - Brady JN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (ETS1 protein, human)
RN  - 0 (Gene Products, tax)
RN  - 0 (Proto-Oncogene Protein c-ets-1)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (RNA, Viral)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.28 (Chloramphenicol O-Acetyltransferase)
RN  - EC 3.1.30.1 (Single-Strand Specific DNA and RNA Endonucleases)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chloramphenicol O-Acetyltransferase
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Gene Expression Regulation, Viral
MH  - Gene Products, tax/*metabolism
MH  - Genes, Reporter
MH  - Human T-lymphotropic virus 1/*genetics
MH  - Molecular Sequence Data
MH  - Moths
MH  - Promoter Regions, Genetic/genetics
MH  - Proto-Oncogene Protein c-ets-1
MH  - Proto-Oncogene Proteins/*metabolism
MH  - Proto-Oncogene Proteins c-ets
MH  - RNA, Viral/metabolism
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Single-Strand Specific DNA and RNA Endonucleases/metabolism
MH  - *Transcription Factors
MH  - *Transcription, Genetic
MH  - Transcriptional Activation
MH  - Transfection
PMC - PMC238194
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
PMCR- 1993/12/01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
PHST- 1993/12/01 00:00 [pmc-release]
AID - 10.1128/JVI.67.12.7307-7316.1993 [doi]
PST - ppublish
SO  - J Virol. 1993 Dec;67(12):7307-16. doi: 10.1128/JVI.67.12.7307-7316.1993.