PMID- 8231628
OWN - NLM
STAT- MEDLINE
DCOM- 19931208
LR  - 20190701
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 53
IP  - 18
DP  - 1993
TI  - Pyrogenic doses of intracerebroventricular interleukin-1 did not induce analgesia 
      in the rat hot-plate or cold-water tail-flick tests.
PG  - 1401-9
AB  - There are a few reports in the literature that cytokines can induce analgesia (5, 
      6, 18). The present study sought to characterize the analgesic effects of 
      intracerebroventricularly (icv) administered interleukin-1 (IL-1) and 
      interferon-alpha (IFN-alpha) in rats. In the cold-water tail-flick test (CWT), 
      latency to tail withdrawal from a -3 degrees C liquid was timed; in the hot-plate 
      test (HP), latency to a rear paw lick or a jump from a 55 degrees C surface was 
      measured. In some experiments, core body temperature was also monitored with a 
      rectal thermistor. In the CWT, human recombinant (hr) IFN-alpha induced a small, 
      statistically significant effect at just one dose (15,000 U icv), but no dose of 
      hr-IL-1 alpha (250-1000 U icv) or hr-IL-1 beta (125-2000 U icv) induced a 
      significant effect at any time point. On the other hand, dose-related increases 
      in body temperature were observed after icv injection of both IL-1 alpha and IL-1 
      beta. The largest hyperthermic effect was a 1.7 (+/- 0.15) degrees C rise 120 min 
      after administration of 1000 U IL-1 beta. In a second analgesic assay, the HP, 
      IL-1 beta was ineffective as well. Since IL-1 alone did not induce analgesia, we 
      tested its capacity to potentiate morphine analgesia. Morphine (5.0 and 10 
      micrograms, icv) induced analgesia in the CWT (32.7 and 61.8% maximum analgesia, 
      respectively); however, there was no significant effect of IL-1 beta on 
      morphine-induced analgesia. In summary, we failed to find an analgesic effect of 
      IL-1, alone or in combination with morphine, at doses which clearly had a 
      physiological effect; this is in contrast to the reports cited above.
FAU - Adams, J U
AU  - Adams JU
AD  - Department of Pharmacology, Temple University School of Medicine, Philadelphia, 
      PA 19140.
FAU - Bussiere, J L
AU  - Bussiere JL
FAU - Geller, E B
AU  - Geller EB
FAU - Adler, M W
AU  - Adler MW
LA  - eng
GR  - DA00376/DA/NIDA NIH HHS/United States
GR  - T32 DA07237/DA/NIDA NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Interleukin-1)
RN  - 0 (Pyrogens)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - *Analgesia
MH  - Animals
MH  - Body Temperature/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Drug Interactions
MH  - Injections, Intraventricular
MH  - Interleukin-1/pharmacology
MH  - Male
MH  - Morphine
MH  - Pain Measurement/*drug effects
MH  - Pyrogens/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 0024-3205(93)90582-N [pii]
AID - 10.1016/0024-3205(93)90582-n [doi]
PST - ppublish
SO  - Life Sci. 1993;53(18):1401-9. doi: 10.1016/0024-3205(93)90582-n.