PMID- 8231731
OWN - NLM
STAT- MEDLINE
DCOM- 19931201
LR  - 20190825
IS  - 0169-328X (Print)
IS  - 0169-328X (Linking)
VI  - 19
IP  - 4
DP  - 1993 Sep
TI  - Rapid increase of BDNF mRNA levels in cortical neurons following spreading 
      depression: regulation by glutamatergic mechanisms independent of seizure 
      activity.
PG  - 277-86
AB  - Levels of mRNA for nerve growth factor (NGF), brain-derived neurotrophic factor 
      (BDNF), neurotrophin-3 (NT-3) and the tyrosine kinase receptors trkB and trkC 
      have been studied using in situ hybridization in the rat brain after topical 
      application of KCl to the cortical surface (which induces spreading depression). 
      Repeated episodes of spreading depression during 2 h caused a rapid and marked 
      increase of BDNF mRNA levels in deep and, in particular, superficial cortical 
      layers of the ipsilateral hemisphere (to 213 and 417% of control, respectively). 
      Maximal levels were reached within 2 h after the cessation of spreading 
      depression and at 24 h BDNF mRNA expression had returned to control values. 
      Levels of BDNF mRNA were unaffected in the hippocampus, in areas outside the 
      cerebral cortex and in the contralateral hemisphere. Furthermore, no change of 
      the expression of mRNA for NGF, NT-3, trkC or the full length trkB receptor was 
      detected at any time point. However, at 2 h after spreading depression there was 
      an increased level (150% of control) in superficial cortical layers of mRNA 
      hybridizing to an oligonucleotide probe detecting both truncated receptors 
      lacking the tyrosine kinase domain and full length trkB receptors. Also one 
      single episode of spreading depression gave rise to a significant increase of 
      cortical BDNF mRNA levels (to 207% of control), which was attenuated (by 61%) 
      after administration of the competitive NMDA receptor antagonist CGS 19755. The 
      results provide evidence that mild brain insults associated with glutamate 
      release and elevated intracellular calcium, such as spreading depression, also in 
      the absence of seizure activity can lead to activation of the BDNF gene in 
      cortical neurons.
FAU - Kokaia, Z
AU  - Kokaia Z
AD  - Department of Neurology, University Hospital, Lund, Sweden.
FAU - Gido, G
AU  - Gido G
FAU - Ringstedt, T
AU  - Ringstedt T
FAU - Bengzon, J
AU  - Bengzon J
FAU - Kokaia, M
AU  - Kokaia M
FAU - Siesjo, B K
AU  - Siesjo BK
FAU - Persson, H
AU  - Persson H
FAU - Lindvall, O
AU  - Lindvall O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res Mol Brain Res
JT  - Brain research. Molecular brain research
JID - 8908640
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Membrane Proteins)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Pipecolic Acids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 4VGJ4A41L2 (selfotel)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Base Sequence
MH  - Brain-Derived Neurotrophic Factor
MH  - Cerebral Cortex/drug effects/metabolism/*physiology
MH  - Cortical Spreading Depression/*physiology
MH  - In Situ Hybridization
MH  - Male
MH  - Membrane Potentials
MH  - Membrane Proteins/biosynthesis
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*biosynthesis
MH  - Nerve Tissue Proteins/*biosynthesis
MH  - Neurons/metabolism/*physiology
MH  - Oligonucleotide Probes
MH  - Parietal Lobe/metabolism/physiology
MH  - Pipecolic Acids/pharmacology
MH  - Protein-Tyrosine Kinases/biosynthesis
MH  - Pyramidal Tracts/metabolism/physiology
MH  - RNA, Messenger/*biosynthesis/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, Ciliary Neurotrophic Factor
MH  - Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
MH  - Seizures/metabolism/*physiopathology
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 0169-328X(93)90126-A [pii]
AID - 10.1016/0169-328x(93)90126-a [doi]
PST - ppublish
SO  - Brain Res Mol Brain Res. 1993 Sep;19(4):277-86. doi: 
      10.1016/0169-328x(93)90126-a.