PMID- 8232587
OWN - NLM
STAT- MEDLINE
DCOM- 19931220
LR  - 20131121
IS  - 0028-0836 (Print)
IS  - 0028-0836 (Linking)
VI  - 366
IP  - 6452
DP  - 1993 Nov 18
TI  - Calmodulin-dependent protein kinase II mediates inactivation of MPF and CSF upon 
      fertilization of Xenopus eggs.
PG  - 270-3
AB  - In vertebrates, unfertilized eggs are arrested at second meiotic metaphase by a 
      cytostatic factor (CSF), an essential component of which is the product of the 
      c-mos proto-oncogene. CSF prevents ubiquitin-dependent degradation of mitotic 
      cyclins and thus inactivation or the M phase-promoting factor (MPF). 
      Fertilization or parthenogenetic activation triggers a transient increase in the 
      cytoplasmic free Ca2+ (reviewed in refs 5 and 6), inactivates both CSF and MPF, 
      and releases eggs from meiotic metaphase arrest. A calmodulin-dependent process 
      is required for cyclin degradation to occur in cell-free extracts prepared from 
      metaphase II-arrested eggs (CSF extracts) when the free Ca2+ concentration is 
      transiently raised in the physiological micromolar range. Here we show that when 
      a constitutively active mutant of calmodulin-dependent protein kinase II (CaM 
      KII) is added to a CSF extract, cyclin degradation and Cdc2 kinase inactivation 
      occur even in the absence of Ca2+, and the extract loses its ability to cause 
      metaphase arrest when transferred into embryos. Furthermore, specific inhibitors 
      of CaM KII prevent cyclin degradation after calcium addition. Finally, the direct 
      microinjection of constitutively active CaM KII into unfertilized eggs 
      inactivates Cdc2 kinase and CSF, even in the absence of a Ca2+ transient. The 
      target for Ca(2+)-calmodulin is thus CaM KII.
FAU - Lorca, T
AU  - Lorca T
AD  - Centre National de la Recherche Scientifique, Montpellier, France.
FAU - Cruzalegui, F H
AU  - Cruzalegui FH
FAU - Fesquet, D
AU  - Fesquet D
FAU - Cavadore, J C
AU  - Cavadore JC
FAU - Mery, J
AU  - Mery J
FAU - Means, A
AU  - Means A
FAU - Doree, M
AU  - Doree M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nature
JT  - Nature
JID - 0410462
RN  - 0 (Cyclins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-mos)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
RN  - EC 2.7.11.22 (CDC2 Protein Kinase)
RN  - EC 2.7.11.22 (Maturation-Promoting Factor)
RN  - SY7Q814VUP (Calcium)
SB  - IM
CIN - Nature. 1993 Nov 18;366(6452):211-2. PMID: 8232578
MH  - Amino Acid Sequence
MH  - Animals
MH  - CDC2 Protein Kinase/metabolism
MH  - Calcium/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/genetics/*metabolism
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Cyclins/metabolism
MH  - Enzyme Activation
MH  - *Fertilization
MH  - Maturation-Promoting Factor/*antagonists & inhibitors
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Myosin-Light-Chain Kinase/metabolism
MH  - Peptide Fragments/metabolism
MH  - Proto-Oncogene Proteins c-mos/*antagonists & inhibitors
MH  - Rats
MH  - Recombinant Proteins/metabolism
MH  - Xenopus
EDAT- 1993/11/18 00:00
MHDA- 2000/03/11 09:00
CRDT- 1993/11/18 00:00
PHST- 1993/11/18 00:00 [pubmed]
PHST- 2000/03/11 09:00 [medline]
PHST- 1993/11/18 00:00 [entrez]
AID - 10.1038/366270a0 [doi]
PST - ppublish
SO  - Nature. 1993 Nov 18;366(6452):270-3. doi: 10.1038/366270a0.