PMID- 8233617
OWN - NLM
STAT- MEDLINE
DCOM- 19931216
LR  - 20071115
IS  - 0048-2951 (Print)
IS  - 0048-2951 (Linking)
VI  - 35 Suppl
DP  - 1993 Jul
TI  - Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum in 
      parasite-host cell interactions.
PG  - 69-72
AB  - Thrombospondin related anonymous protein (TRAP) of Plasmodium falciparum is 
      characterized by the presence of an amino acid motif based on the sequence 
      Trp-Ser-Pro-Cys-Ser-Val-Thr-Cys-Gly (WSPCSVTCG) that is found in a growing family 
      of proteins. The sequence WSPCSVTCG is considered to confer 
      sulpho-galactosyl-cerebroside (sulphatide) binding properties to antistasin, TSP, 
      CS protein and properdin. The observation that TRAP is localized both on the 
      micronemes and on the surface of P. falciparum sporozoites would suggest a role 
      played by TRAP, and its putative sulphated glycoconjugates binding motif, in the 
      recognition and/or entry of hepatocytes by the sporozoite. Our results indicated 
      that TRAP constructs, expressed in E. coli, bind to 
      sulpho-galactosyl-cerebrosides (sulphatides) and to the surface of HepG2 cells 
      using the conserved amino acid motif WSPCSVTCG. Antisera raised against TRAP 
      constructs inhibited sporozoite invasion of HepG2 cells thus suggesting, thus, 
      that TRAP may be one of the parasite-encoded molecules implicated in the 
      sporozoite invasion of hepatocytes. Moreover, the possibility that TRAP 
      antibodies may be relevant in malaria immunity is supported by the results 
      obtained in a prospective study conducted in a malaria endemic area. In 
      adolescents, the presence of TRAP antibodies, before malaria transmission, 
      correlated positively with the control of parasite density.
FAU - Muller, H M
AU  - Muller HM
AD  - Istituto di Parassitologia, Universita di Roma La Sapienza, Italy.
FAU - Scarselli, E
AU  - Scarselli E
FAU - Crisanti, A
AU  - Crisanti A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Parassitologia
JT  - Parassitologia
JID - 0413724
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Sulfoglycosphingolipids)
RN  - 120300-02-9 (thrombospondin-related adhesive protein, protozoan)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antibodies, Protozoan/blood
MH  - Binding Sites
MH  - Child
MH  - Child, Preschool
MH  - Host-Parasite Interactions
MH  - Humans
MH  - Liver/parasitology
MH  - Malaria, Falciparum/immunology
MH  - Molecular Sequence Data
MH  - Plasmodium falciparum/immunology/*physiology
MH  - Protein Binding
MH  - Protozoan Proteins/immunology/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Sulfoglycosphingolipids/*metabolism
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 1993/07/01 00:00
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
PHST- 1993/07/01 00:00 [entrez]
PST - ppublish
SO  - Parassitologia. 1993 Jul;35 Suppl:69-72.