PMID- 8236798
OWN - NLM
STAT- MEDLINE
DCOM- 19931214
LR  - 20190830
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 37
IP  - 3-4
DP  - 1993 Aug
TI  - Production and characterization of mouse monoclonal antibodies directed against 
      canine IgE and IgG.
PG  - 183-99
AB  - Immediate-type hypersensitivity reactions have been studied infrequently in dogs, 
      in part because of limited availability of antisera specific for canine IgE. A 
      series of murine hybridoma cell lines were prepared, that produced monoclonal 
      antibodies (MAb) with specificity for canine immunoglobulin E (IgE) and IgG. The 
      MAb were tested for their ability to induce a reverse cutaneous anaphylaxis 
      reaction in dog skin, to neutralize the Prausnitz-Kustner reactivity of atopic 
      dog serum, to serve as a ligand in immunoaffinity chromatography, and to bind to 
      IgE and other Ig subclasses in several ELISA systems. Some of the MAb produced 
      were found to be specific for canine IgE. Other MAb recognized common or similar 
      determinants on IgE and IgG, or on IgG and IgM, though with apparently differing 
      affinities. Heat or acid treatment of canine IgE abolished most, but not all, of 
      the reactivity with the anti-IgE MAb. These MAb will be useful for further study 
      of IgE-mediated phenomena in the dog.
FAU - DeBoer, D J
AU  - DeBoer DJ
AD  - Department of Medical Sciences, School of Veterinary Medicine, University of 
      Wisconsin, Madison 53706.
FAU - Ewing, K M
AU  - Ewing KM
FAU - Schultz, K T
AU  - Schultz KT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Immunoglobulin G)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antibody Specificity/immunology
MH  - Cell Line
MH  - Chromatography, Affinity
MH  - Dogs/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Hybridomas/immunology
MH  - Immunoglobulin E/*biosynthesis
MH  - Immunoglobulin G/*biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Passive Cutaneous Anaphylaxis/*immunology
EDAT- 1993/08/01 00:00
MHDA- 1993/08/01 00:01
CRDT- 1993/08/01 00:00
PHST- 1993/08/01 00:00 [pubmed]
PHST- 1993/08/01 00:01 [medline]
PHST- 1993/08/01 00:00 [entrez]
AID - 0165-2427(93)90193-8 [pii]
AID - 10.1016/0165-2427(93)90193-8 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 1993 Aug;37(3-4):183-99. doi: 
      10.1016/0165-2427(93)90193-8.