PMID- 8239070 OWN - NLM STAT- MEDLINE DCOM- 19931201 LR - 20190914 IS - 0268-9146 (Print) IS - 0268-9146 (Linking) VI - 24 IP - 4 DP - 1993 Aug TI - Preparation of chromosome-specific paints and complete assignment of chromosomes in the pig flow karyotype. PG - 261-7 AB - Sorted chromosomes from each of the 20 clusters of the male porcine bivariate flow karyotype were amplified and biotinylated using DOP-PCR. The chromosomes comprising each cluster were identified by fluorescence in situ hybridization (FISH) of the 20 probes to R-banded male pig metaphase spreads. A standard flow karyotype for the pig is presented. FAU - Langford, C F AU - Langford CF AD - AFRC Institute of Animal Physiology & Genetics Research, Babraham Research Station, Babraham, UK. FAU - Telenius, H AU - Telenius H FAU - Miller, N G AU - Miller NG FAU - Thomsen, P D AU - Thomsen PD FAU - Tucker, E M AU - Tucker EM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Anim Genet JT - Animal genetics JID - 8605704 RN - 0 (DNA Primers) RN - 0 (Fluorescent Dyes) RN - 6SO6U10H04 (Biotin) SB - IM MH - Animals MH - Base Sequence MH - Biotin MH - Cells, Cultured MH - Chromosomes/ultrastructure MH - DNA Primers MH - Female MH - Flow Cytometry/veterinary MH - Fluorescent Dyes MH - In Situ Hybridization, Fluorescence/*veterinary MH - Karyotyping/*veterinary MH - Male MH - Molecular Sequence Data MH - Polymerase Chain Reaction/veterinary MH - Swine/*genetics EDAT- 1993/08/01 00:00 MHDA- 1993/08/01 00:01 CRDT- 1993/08/01 00:00 PHST- 1993/08/01 00:00 [pubmed] PHST- 1993/08/01 00:01 [medline] PHST- 1993/08/01 00:00 [entrez] AID - 10.1111/j.1365-2052.1993.tb00309.x [doi] PST - ppublish SO - Anim Genet. 1993 Aug;24(4):261-7. doi: 10.1111/j.1365-2052.1993.tb00309.x.