PMID- 8240787
OWN - NLM
STAT- MEDLINE
DCOM- 19931228
LR  - 20190914
IS  - 0903-4641 (Print)
IS  - 0903-4641 (Linking)
VI  - 101
IP  - 9
DP  - 1993 Sep
TI  - Multiplex polymerase chain reaction for detection of genes for Staphylococcus 
      aureus thermonuclease and methicillin resistance and correlation with oxacillin 
      resistance.
PG  - 681-8
AB  - A multiplex polymerase chain reaction (mPCR) was used for simultaneous 
      amplification of the staphylococcal nuc gene, encoding the thermostable nuclease 
      (TNase), and the mecA gene, encoding the penicillin-binding protein 2a which is 
      associated with staphylococcal methicillin resistance. A total of 219 
      staphylococcal strains were tested and the mPCR data were compared with coagulase 
      production and in vitro oxacillin susceptibility. The agreement was 100% for 
      coagulase production and nuc amplification, and 97.7%, 96.8 and 97.3% for mecA 
      amplification and oxacillin resistance tested with MIC determination, disk 
      diffusion and agar screen methods, respectively. Discrepant results were due to 
      non-S. aureus isolates with borderline MICs of oxacillin (1-8 micrograms/ml). In 
      a pilot test the mPCR simultaneously amplified both genes of staphylococci in 
      blood cultures. This mPCR is a rapid and reliable method for single-step 
      identification of cultures of MRSA and may prove to be useful for direct 
      application on clinical specimens.
FAU - Brakstad, O G
AU  - Brakstad OG
AD  - Applied Chemistry Division, SINTEF, Trondheim, Norway.
FAU - Maeland, J A
AU  - Maeland JA
FAU - Tveten, Y
AU  - Tveten Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
EIN - APMIS 1994 Mar;102(3):241
GS  - mecA
GS  - nuc
MH  - DNA Primers
MH  - DNA, Bacterial/analysis/isolation & purification
MH  - Drug Resistance, Microbial/*genetics
MH  - Electrophoresis, Agar Gel
MH  - *Genes, Bacterial
MH  - Methicillin Resistance/*genetics
MH  - Microbial Sensitivity Tests
MH  - Micrococcal Nuclease/*genetics
MH  - Oxacillin/*toxicity
MH  - Polymerase Chain Reaction/*methods
MH  - Staphylococcus/genetics
MH  - Staphylococcus aureus/drug effects/*enzymology/*genetics
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1111/j.1699-0463.1993.tb00165.x [doi]
PST - ppublish
SO  - APMIS. 1993 Sep;101(9):681-8. doi: 10.1111/j.1699-0463.1993.tb00165.x.