PMID- 8240799
OWN - NLM
STAT- MEDLINE
DCOM- 19940106
LR  - 20231213
IS  - 0958-0670 (Print)
IS  - 0958-0670 (Linking)
VI  - 78
IP  - 5
DP  - 1993 Sep
TI  - Volume-sensitive KCl co-transport and taurine fluxes in horse red blood cells.
PG  - 685-95
AB  - Potassium (using 86Rb+ as a tracer), amino acid and taurine fluxes were measured 
      in horse red blood cells (RBCs). No volume-sensitive component of alanine and 
      glycine transport was observed, and although volume-sensitive taurine fluxes were 
      observed in most animals, their absolute magnitudes were small. K+ fluxes, 
      however, were shown to be particularly volume sensitive; they were stimulated by 
      cell swelling and inhibited by cell shrinkage. Sizeable fluxes were present at 
      normal cell volumes. The volume-sensitive K+ flux was Cl- dependent and was 
      abolished by Cl- replacement with methylsulphate. The Cl(-)-dependent K+ fluxes 
      in horse red blood cells were stimulated by lowering in external pH to 6.9 and by 
      treatment with the sulphydryl-reacting agent, N-ethylmaleimide. They were 
      inhibited by the potent K(+)-Cl- co-transport inhibitor, DIOA, 
      ([(dihydroindenyl)oxy]alkanoic acid) but were insensitive to the Na(+)-K(+)-Cl- 
      co-transport inhibitors, frusemide and bumetanide. A Cl- channel inhibitor, 
      5-nitro-2-(phenylpropyl-amino)-benzoate (NPPB), produced partial inhibition. 
      These results suggest that regulatory volume decrease in horse red blood cells is 
      achieved predominantly by volume-sensitive K+ efflux mediated via a K(+)-Cl- 
      co-transport system with similar properties to those observed in the red blood 
      cells of other species. The significance of these findings and their rheological 
      consequences are discussed.
FAU - Gibson, J S
AU  - Gibson JS
AD  - Department of Veterinary Medicine, University of Cambridge.
FAU - Ellory, J C
AU  - Ellory JC
FAU - Culliford, S J
AU  - Culliford SJ
FAU - Fincham, D A
AU  - Fincham DA
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Exp Physiol
JT  - Experimental physiology
JID - 9002940
RN  - 0 (((dihydroindenyl)oxy)alkanoic acid)
RN  - 0 (Carboxylic Acids)
RN  - 0 (Carrier Proteins)
RN  - 0 (Indenes)
RN  - 0 (Symporters)
RN  - 0Y2S3XUQ5H (Bumetanide)
RN  - 1EQV5MLY3D (Taurine)
RN  - 7LXU5N7ZO5 (Furosemide)
RN  - O3C74ACM9V (Ethylmaleimide)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Animals
MH  - Bumetanide/pharmacology
MH  - Carboxylic Acids/pharmacology
MH  - Carrier Proteins/analysis/*physiology
MH  - Erythrocyte Volume/*physiology
MH  - Erythrocytes/chemistry/*metabolism/*physiology
MH  - Ethylmaleimide/pharmacology
MH  - Female
MH  - Furosemide/pharmacology
MH  - Horses/*blood
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Indenes/pharmacology
MH  - Male
MH  - Potassium/metabolism
MH  - *Symporters
MH  - Taurine/*metabolism
MH  - K Cl- Cotransporters
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - 10.1113/expphysiol.1993.sp003716 [doi]
PST - ppublish
SO  - Exp Physiol. 1993 Sep;78(5):685-95. doi: 10.1113/expphysiol.1993.sp003716.