PMID- 8240982
OWN - NLM
STAT- MEDLINE
DCOM- 19940106
LR  - 20190830
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 46
IP  - 5
DP  - 1993 Nov
TI  - Estrogen and androgen receptor mediated stimulation and inhibition of 
      proliferation by androst-5-ene-3 beta,17 beta-diol in human mammary cancer cells.
PG  - 597-603
AB  - Androst-5-ene-3 beta,17 beta-diol (ADIOL) and 5 alpha-androstane-3 beta,17 
      beta-diol (5 alpha A), which are metabolites of dehydroepiandrosterone and 
      dihydrotestosterone, are known to have estrogenic properties. This study 
      reevaluates the estrogenic effects of ADIOL and 5 alpha A in MCF-7 cells and 
      demonstrates additionally androgen-like inhibitory properties of these compounds 
      in human hormone-dependent mammary cancer cells. ADIOL and 5 alpha A (10-100 nM) 
      stimulate the proliferation of estrogen-sensitive MCF-7 cells. Binding assays 
      with the estrogen receptor and inhibition of stimulation with the antiestrogen 
      tamoxifen support the involvement of the estrogen receptor. On the other hand, 
      the mammary cancer cell line MFM-223 is strongly inhibited by ADIOL and 5 alpha A 
      in the same concentration range. This cell line is androgen receptor positive and 
      is inhibited by androgens, but unresponsive to estrogens and progestins. The 
      inhibitory effects of ADIOL and 5 alpha A in MFM-223 cells are mediated by the 
      androgen receptor as demonstrated by receptor studies and competition experiments 
      with hormone antagonists. ADIOL and 5 alpha A thus possess estrogen- and 
      androgen-like properties and can stimulate or inhibit proliferation of human 
      mammary cancer cells. The reactions of mammary cancer cells to these steroids 
      depend on the receptor content and the growth properties of the individual cell 
      line.
FAU - Hackenberg, R
AU  - Hackenberg R
AD  - Zentrum fur Frauenheilkunde und Geburtshilfe, Philipps Universitat, Marburg, 
      Germany.
FAU - Turgetto, I
AU  - Turgetto I
FAU - Filmer, A
AU  - Filmer A
FAU - Schulz, K D
AU  - Schulz KD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, Estrogen)
RN  - 25126-76-5 (Androstane-3,17-diol)
RN  - 2C323EGI97 (Metribolone)
RN  - 95PS51EMXY (Androstenediol)
SB  - IM
MH  - Androstane-3,17-diol/pharmacology
MH  - Androstenediol/*pharmacology
MH  - Binding, Competitive
MH  - Breast Neoplasms/*metabolism
MH  - Cell Division/drug effects
MH  - Female
MH  - Humans
MH  - Metribolone/metabolism
MH  - Receptors, Androgen/*metabolism
MH  - Receptors, Estrogen/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 1993/11/01 00:00
MHDA- 1993/11/01 00:01
CRDT- 1993/11/01 00:00
PHST- 1993/11/01 00:00 [pubmed]
PHST- 1993/11/01 00:01 [medline]
PHST- 1993/11/01 00:00 [entrez]
AID - 10.1016/0960-0760(93)90187-2 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 1993 Nov;46(5):597-603. doi: 
      10.1016/0960-0760(93)90187-2.