PMID- 8242385
OWN - NLM
STAT- MEDLINE
DCOM- 19940106
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 622
IP  - 1-2
DP  - 1993 Sep 17
TI  - Distribution of trkB tyrosine kinase immunoreactivity in the rat central nervous 
      system.
PG  - 63-70
AB  - Recent evidence suggests that trkB tyrosine kinase is a high affinity receptor 
      for brain-derived neurotrophic factor (BDNF). BDNF can act as a survival factor 
      for several neuronal subgroups and its mRNA is distributed widely throughout the 
      central nervous system. However, the functional targets of BDNF are poorly 
      defined. We have used immunochemical and immunohistochemical techniques to 
      determine the regional distribution and cellular localization of trkB tyrosine 
      kinase-like immunoreactivity. The staining pattern indicates that the trkB-like 
      antigen is widely distributed and present within both glia and neurons. 
      Astrocytes were the most intensively labelled but many neuronal populations were 
      also stained. In some regions including brain stem, spinal cord, hippocampus and 
      diagonal band of Broca, neurons were stained at varying intensities. In other 
      areas such as the cortex of the forebrain and amygdaloid nucleus, the stain was 
      intense but diffuse, preventing positive identification of the cell types 
      involved. Immunoblot results indicated two separate protein bands in all brain 
      and spinal cord regions examined, of molecular weights 145 and 85 kDa, 
      respectively. These findings aid the definition of neuronal and glial 
      subpopulations of the central nervous system that may utilize BDNF.
FAU - Zhou, X F
AU  - Zhou XF
AD  - Department of Physiology, Flinders University of South Australia, Bedford Park.
FAU - Parada, L F
AU  - Parada LF
FAU - Soppet, D
AU  - Soppet D
FAU - Rush, R A
AU  - Rush RA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptor, Ciliary Neurotrophic Factor)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Central Nervous System/*enzymology
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Membrane Proteins/*analysis
MH  - Protein-Tyrosine Kinases/*analysis
MH  - Rats
MH  - Receptor, Ciliary Neurotrophic Factor
EDAT- 1993/09/17 00:00
MHDA- 1993/09/17 00:01
CRDT- 1993/09/17 00:00
PHST- 1993/09/17 00:00 [pubmed]
PHST- 1993/09/17 00:01 [medline]
PHST- 1993/09/17 00:00 [entrez]
AID - 0006-8993(93)90802-T [pii]
AID - 10.1016/0006-8993(93)90802-t [doi]
PST - ppublish
SO  - Brain Res. 1993 Sep 17;622(1-2):63-70. doi: 10.1016/0006-8993(93)90802-t.