PMID- 8242591
OWN - NLM
STAT- MEDLINE
DCOM- 19931230
LR  - 20191210
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 70
IP  - 2
DP  - 1993 Oct 15
TI  - Cell lineage involvement in four patients with myelodysplastic syndrome and 
      t(1;7) or trisomy 8 studied by simultaneous immunophenotyping and fluorescence in 
      situ hybridization.
PG  - 120-4
AB  - Four patients with myelodysplastic syndrome (MDS), one with t(1;7) and three with 
      trisomy 8, were studied by immunophenotyping and fluorescence in situ 
      hybridization (FISH) to assess cell lineage involvement. The t(1;7) was detected 
      using a biotin-labeled chromosome 1 centromere-specific DNA probe. This 
      aberration was present in CD34-positive stem cells, the erythroid cell lineage 
      (GPA+), and the granulocytic/monocytic (CD13+ and CD64+) cell lineages. We were 
      not able to demonstrate the abnormality in the lymphoid cell lineages. In the 
      patients with trisomy 8, the aberration was detected with chromosome 8 
      centromere-specific DNA probe or by chromosome in situ suppression hybridization 
      (CISS) with a chromosome 8-specific library probe. The trisomy was detected in 
      stem cells, erythroid precursor cells, megakaryocytes, and 
      granulocytes/monocytes. In these MDS patients, the chromosome aberrations appear 
      to occur only in cells of myeloid lineage.
FAU - Nylund, S J
AU  - Nylund SJ
AD  - Department of Medical Genetics, University of Helsinki, Finland.
FAU - Verbeek, W
AU  - Verbeek W
FAU - Larramendy, M L
AU  - Larramendy ML
FAU - Ruutu, T
AU  - Ruutu T
FAU - Heinonen, K
AU  - Heinonen K
FAU - Hallman, H
AU  - Hallman H
FAU - Knuutila, S
AU  - Knuutila S
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Antigens, CD)
RN  - 0 (DNA Probes)
RN  - 0 (Glycophorins)
SB  - IM
MH  - Aged
MH  - Antigens, CD/biosynthesis
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 7
MH  - *Chromosomes, Human, Pair 8
MH  - DNA Probes
MH  - Erythroid Precursor Cells/chemistry/ultrastructure
MH  - Female
MH  - Gene Library
MH  - Glycophorins/analysis
MH  - Granulocytes/immunology/ultrastructure
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Megakaryocytes/ultrastructure
MH  - Middle Aged
MH  - Monocytes/immunology/ultrastructure
MH  - Myelodysplastic Syndromes/*genetics/immunology
MH  - Stem Cells/immunology/ultrastructure
MH  - *Translocation, Genetic
MH  - *Trisomy
EDAT- 1993/10/15 00:00
MHDA- 1993/10/15 00:01
CRDT- 1993/10/15 00:00
PHST- 1993/10/15 00:00 [pubmed]
PHST- 1993/10/15 00:01 [medline]
PHST- 1993/10/15 00:00 [entrez]
AID - 0165-4608(93)90180-T [pii]
AID - 10.1016/0165-4608(93)90180-t [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1993 Oct 15;70(2):120-4. doi: 
      10.1016/0165-4608(93)90180-t.