PMID- 8243273
OWN - NLM
STAT- MEDLINE
DCOM- 19940106
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 133
IP  - 6
DP  - 1993 Dec
TI  - Effects of tumor necrosis factor-alpha on luteinizing hormone-stimulated prorenin 
      production by bovine ovarian thecal cells in vitro.
PG  - 2515-22
AB  - Investigations have been carried out to determine if the cytokine tumor necrosis 
      factor-alpha (TNF alpha), a putative intraovarian regulator, plays a role in the 
      regulation of the ovarian prorenin-renin-angiotensin system. Addition of TNF 
      alpha to cultured bovine thecal cells resulted in a dose-dependent inhibition of 
      LH- or 8-bromo-cAMP-stimulated production of prorenin and renin by the cells in a 
      noncytotoxic manner. No clear inhibitory effect on progesterone production was 
      noted. There was no inhibition of LH- or forskolin-stimulated cAMP formation by 
      TNF alpha. The time-course experiment with TNF alpha revealed that the synthesis, 
      rather than the secretion, of prorenin was inhibited. Also, it was evident that 
      to observe a maximal inhibitory effect, it was necessary to add TNF alpha either 
      before or together with LH. With the increasing delay in the addition of TNF 
      alpha relative to the time of addition of LH, the extent of inhibition gradually 
      decreased, and TNF alpha added 6 h after the addition of LH failed to produce any 
      inhibitory effect. The results obtained permit us to conclude that TNF alpha can 
      counterregulate LH-stimulated prorenin production by thecal cells in culture. The 
      TNF alpha-induced lesion appears to be located at an early step of the 
      biosynthetic pathway of prorenin, which is distal to the activation of LH 
      receptor-coupled adenylate cyclase. Thus, this cytokine appears to be an 
      important intraovarian regulator of prorenin production, a process that is under 
      the stimulatory control of the pituitary gonadotropin.
FAU - Brunswig-Spickenheier, B
AU  - Brunswig-Spickenheier B
AD  - Institute for Hormone and Fertility Research, University of Hamburg, Germany.
FAU - Mukhopadhyay, A K
AU  - Mukhopadhyay AK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Enzyme Precursors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 1F7A44V6OU (Colforsin)
RN  - 23583-48-4 (8-Bromo Cyclic Adenosine Monophosphate)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 3.4.23.15 (Renin)
SB  - IM
MH  - 8-Bromo Cyclic Adenosine Monophosphate/pharmacology
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Colforsin/pharmacology
MH  - Enzyme Precursors/antagonists & inhibitors/*biosynthesis
MH  - Female
MH  - Luteinizing Hormone/*pharmacology
MH  - Progesterone/antagonists & inhibitors/biosynthesis
MH  - Renin/antagonists & inhibitors/*biosynthesis/pharmacology
MH  - Theca Cells/*metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1993/12/01 00:00
MHDA- 1993/12/01 00:01
CRDT- 1993/12/01 00:00
PHST- 1993/12/01 00:00 [pubmed]
PHST- 1993/12/01 00:01 [medline]
PHST- 1993/12/01 00:00 [entrez]
AID - 10.1210/endo.133.6.8243273 [doi]
PST - ppublish
SO  - Endocrinology. 1993 Dec;133(6):2515-22. doi: 10.1210/endo.133.6.8243273.