PMID- 8244385
OWN - NLM
STAT- MEDLINE
DCOM- 19931229
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 17
IP  - 3
DP  - 1993 Sep
TI  - Characterization of two chromosome 12 cosmid libraries and development of STSs 
      from cosmids mapped by FISH.
PG  - 682-93
AB  - We have constructed and characterized two related human chromosome 12-specific 
      cosmid libraries. DNA from flow-sorted chromosomes from a somatic cell hybrid was 
      cloned into a cosmid vector. Approximately 61% of the cosmids in the nearly 
      26,200 member arrayed libraries (LL12NC01 and LL12NC02) contain human DNA 
      inserts, and 31% of the cosmids derived from human DNA contain CA repeats. One 
      hundred and fifty-two cosmids isolated from the libraries have been mapped by 
      fluorescence in situ hybridization (FISH). Cosmids containing human DNA inserts 
      were localized by FISH exclusively to chromosome 12, confirming the chromosomal 
      specificity of the libraries. The cosmids have been localized to all parts of 
      this chromosome, although some regions are more highly represented than others. 
      Partial sequence information was obtained from 44 mapped cosmids, and 
      oligonucleotide primer pairs were synthesized that define unique sequence tagged 
      sites (STSs). These mapped cosmids, and unique STSs derived from them, provide a 
      set of useful clones and primer pairs for screening YAC libraries and developing 
      contigs centered on regions of interest within chromosome 12. In addition, 120 of 
      the mapped cosmids contain CA repeats, and thus they also provide a useful 
      resource for defining highly polymorphic simple tandem repeat elements that serve 
      as genetic markers for linkage analysis and disease gene localization.
FAU - Montgomery, K T
AU  - Montgomery KT
AD  - Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 
      10461.
FAU - LeBlanc, J M
AU  - LeBlanc JM
FAU - Tsai, P
AU  - Tsai P
FAU - McNinch, J S
AU  - McNinch JS
FAU - Ward, D C
AU  - Ward DC
FAU - de Jong, P J
AU  - de Jong PJ
FAU - Kucherlapati, R
AU  - Kucherlapati R
FAU - Krauter, K S
AU  - Krauter KS
LA  - eng
GR  - CA39553/CA/NCI NIH HHS/United States
GR  - GM33943/GM/NIGMS NIH HHS/United States
GR  - HG00670/HG/NHGRI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast
MH  - *Chromosomes, Human, Pair 12
MH  - Cosmids/*genetics
MH  - DNA/genetics
MH  - *Gene Library
MH  - Genetic Markers
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Repetitive Sequences, Nucleic Acid
MH  - *Sequence Tagged Sites
EDAT- 1993/09/01 00:00
MHDA- 1993/09/01 00:01
CRDT- 1993/09/01 00:00
PHST- 1993/09/01 00:00 [pubmed]
PHST- 1993/09/01 00:01 [medline]
PHST- 1993/09/01 00:00 [entrez]
AID - S0888-7543(83)71390-X [pii]
AID - 10.1006/geno.1993.1390 [doi]
PST - ppublish
SO  - Genomics. 1993 Sep;17(3):682-93. doi: 10.1006/geno.1993.1390.